Application Of Porous Zein Scaffolds Loaded With Magnetic Mesoporous Silica Nanoparticles In Reconstruction Of Bone Defects

Objective: The purpose of this study is to explore the role and mechanism of zein scaffolds loaded with mesoporous silicon-coated magnetic nanoparticles(M-MSNs)in reconstruction of bone defects.Methods: 1.Porous zein scaffolds loaded with M-MSNs were synthesized.The level of M-MSNs released was observed.2.Cell viability were assessed using CCK-8,live/dead assay and Phalloidin&DAPI staining.Osteogenic differentiation were assessed using alkaline phosphatase staining,alizarin red staining,and q RT-PCR.3.The scaffolds were inserted into the rat tibial segmental defect models for 12 weeks.The samples was evaluated by micro-CT,HE staining and Masson staining.4.The rabbit tibial distraction osteogenesis model was used.The scaffolds were inserted into the bone marrow.X-rays,micro-computed tomography(Micro-CT),mechanical testing,PCR,HE staining,Massons staining were used to evaluate new bone regeneration.Results: Porous zein scaffolds loaded with M-MSNs had good biocompatibility.The prepared scaffolds promoted the osteogenic differentiation of bone marrow-derived mesenchymal stem cells(BMSCs)in vitro.The results of tibial defect model showed that zein scaffolds loaded with M-MSNs could promote new bone regeneration in vivo.The rabbit tibial distraction osteogenesis model showed that the prepared scaffolds could promote the regeneration and consolidation of new bone in the distraction zone.Conclusion: Porous zein scaffolds loaded with M-MSNs have good biocompatibility,promoting bone regeneration and consolidation.It might be a choice for tissue engineering.