The Research Of The Effects Of Juli Sanjie Pill On Proliferation, Apoptosis, Related Factors Expression Levels In Uterine Leiomyoma Cells

(Hunan College of Traditional Chinese Medicine, Changsha,410007)Objects:To explore the influence of Juli Sanjie Pill (JSP)on proliferation, apoptosis, progesterone receptor A (PR-A), EGF, EGFR expression level of Uterine Leiomyoma Cells (ULCs), observe the Effective parts, and provide a more scientific basis on Drug screening and streamlining the prescription.Method:1. Uterine Leiomyoma Cells (ULCs) were digested with collagenase in primary culture.2. The chemical composition of groups JSP was extracted by modern technology.3. MTT, Flow cytometry, immunocytochemistry, immunofluorescence and euzymelinked immunosorbent assay (ELISA) were performed to observe the expression and changes on proliferation, apoptosis, PR-A, EGF, EGFR of ULCs, which were interved by JSP.Result:1. ULCs were successfully cultured.2.1) Compared with the blank group, OD of the alcohol extraction group. the water extraction and alcohol precipitation liquid group、the sediments from the water and alcohol group and the volatile oil group of JSP were significantly lower and their difference which was a dose-dependent was statistically significant (p<0.05).2) There was significant difference between the blank group and the alcohol extraction group in the rate of apoptosis (p<0.05), and there was no statistical significance in other extracts group of JPS (p>0.05). However, compared with the blank group, the rate of apoptosis in the four group of JPS grown more quickly.3) The difference was significant difference between the blank group and the four group of JPS in the MOD expression of ULCs where PR-A was positive (p<0.05).4) Compared with the blank group, the secretion of EGF in the high concentrations of the extraction group、the water extraction and alcohol precipitation liquid group and the volatile oil group of JSP were significantly decreased and the difference was statistically significant (p<0.05), but there was no significant difference between the blank group and the sediments from the water and alcohol group (p>0.05).5) The four group of JPS were significantly lower EGFR expression comparing with the blank group (p<0.05), and the EGFR expression of the two volatile oil group was significantly different (p<0.05).3. Compared with the RU486 group, EGFR of the alcohol extraction group、the water extraction and alcohol precipitation liquid group、the sediments from the water and alcohol group and the volatile oil group of JSP were significantly lower (p<0.05).Conclusion:It indicates that it is one of mechanisms for Juli Sanjie Pill (JSP) to treat Uterine Leiomyoma to induce apoptosis of the ULCs, make it spontaneous regression, make PR-A、EGFR and EGF fall in ULCs. The role of the extraction group was more significant.