Proteomic analysis of proinflammatory cell death by capillary electrophoresis and mass spectrometry

The extent to which researchers are able to elucidate the molecular mechanisms of biological processes is directly dependent on their ability to accurately identify and quantify the proteins present in a given sample. The contribution of bioanalytical chemistry to the field of proteomics is the development of tools for rapid and sensitive identification and quantification of gene products. The work presented here will discuss the application of two such techniques---capillary electrophoresis with laser-induced fluorescence detection and isotope coded affinity tagging mass spectrometry---to the study of proinflammatory cell death.