GO Analysis And Network Construction Of Abnormally Expressed Genes And MicroRNAs In Cancers

Cancer is a serious crisis to human’s life and health,despite of the fact that researchers have paid so much efforts on it ever since cancer came into people’s understanding.Still,there is no cure for cancer.Generally speaking,cancer is usually be found along with genetic mutations.Most of the cancer cases occurred from the gene mutation because of changes of environment including living habits and virus/radiation hazards.The rest were consequences of inheritance from parents.In any case,it is a general consensus that cancer is closely related to the abnormally expression of genes in cells.Activation of oncogene of inhibition of tumor suppressor gene are both potential trigger events of carcinogenesis.In recent years,a novel genetic molecule,microRNA(miRNA),which attracts extensive attentions was located.A microRNA is endogenous,20~22 nucleartides-long single-strand noncoding RNA.With increasing understanding of miRNA,its critical role in biological process and diseases development including cancers gains awareness.Through complementary base pairing,a mature miRNA molecule can bind to its target genes’ messenger RNA molecules.As a consequence the translation is obstructed or depressed.As a result a miRNA can serves as a posttranscriptional regulator of specific genes.More and more researches are uncovering the significance of miRNAs during cancer development.Lots of reports of comparisons between normal and tumor tissues show that genes and miRNAs tend to express abnormally in cancer tissues.The expression was found to be up or down regulated.Other cases show losses or mutations.These abnormally expressed genes and miRNAs can be utilized as marker of cancers.Base on the targeting relations between miRNAs and genes,regulating network can be constructed.The regulating relation between genes and miRNAs could be signaling pathway which is likely to be involved in cancer development.Logically,it is meaningful to use the network to extract pathways and identify core genes and miRNAs to make a better understanding of cancer.The current research was initiated to take analysis on cancer related genes and miRNAs with biological significance taken into consideration.Experimentally validated abnormally expressed genes and miRNAs were extracted from articles and authoritative databases including miR2 Diease and PhenomiR2.0.Experimentally validated miRNA-target relations were acquired from Tarbase 8.0 and miRTarbase.With the Help of The Database for Annotation,Visualization and Integrated Discovery(DAVID)and ClueGO,Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis were applied on the nodes within the network.Gene annotation and enrichment were conducted so that the genes were connected with specific biological process and cancers and the activation and inhibition between nodes were achieved.A ranking model was proposed to assess the realtions between genes and specific cancers.Through regulatory network construction the interactions among miRNA,gene and biological process were investigated.Abnormally expressed genes and miRNAs involved in fifteen cancers were taken into consideration.The comprehensive analysis of them were enriched.More related genes were produced.The genes were coupling with miRNAs and so that networks were built.In the independent research of cancers,the DAVID Annotation Chart made genes annotated and classified into biologycial themes.Notable gene clusters were retrieved and miRNA regulating networks were consturcted.