Application Of Mussel-derived Biomaterials In Drug Delivery And Tissue Repair

BackgroundThe DOPA molecules,derived from mussel adhesive proteins,have strong adhesion ability and well biocompatibility.They are widely applied in surface modification,tissue engineering,drug delivery and biological adhesives.In addition,mussel shells,because of its regular structure,and possessing many unique characteristics such as light weight,high strength and high toughness,is a promising natural biomaterials used in tissue engineering.In this paper,making the use of super adhesion and reducibility properties of DOPA,and structural characteristics of the mussel shells,we constructed three kinds of new materials,and launched some application in the nano-drug carrier,skin trauma and myocardial tissue engineering.ObjectiveTo construct of a series of mussel-derived biological materials,promote wide application in the mussel adhesive proteins and mussel shell-derived scaffolds.Methods1.Constructinging a type of Fe3O4 nano-drug carrier system,we used polydopamine as "super glue" to conjugate DOX onto the surface of MNPs,select folic acid as tumor-specific targeting.The Schiff-base bonds between polydopamine and chitosan would automatically break due to acid microenvironment of cancer tumor and released DOX.The characterization,performance,and tumor killing effect of the constructed nanoparticles was preliminarily investigated.2.Based on the acid-base digestion,a new mussel shell-derived wound dressing with high elastic and porous structure was constructed.Its role in the process of trauma repair,angiogenesis,collagen deposition and re-epithelialization was detected.3.Using mussel shell-derived wound dressing as scaffold material,making use of reduction and strong adhesion property of polydopamine,a kind of conductive mussel-shell derived cardiac patch material was fabricated.In vitro,the effects of cardiomyocyte maturation and electrical coupling were examined,and the effect of myocardial injury on myocardial repair and cardiac function improvement in myocardial infarction rats was verified in vivo.Results1.The mussel shell-derived chitosan film was soft,toughness,elastic and porous.The compatible chitosan dressing can promote the healing of skin wound when it is used to repair the full layer skin defect.Neovascularization,the newborn epithelium,the collagen and granulation tissue generation and skin accessory organ regeneration was promoted.2.The superparamagnetic magnetic targeting drug loaded nanoparticles based on the polydopamine,exhibitting with excellent property like uniform particle size,good biocompatibility,strong stability and passive targeting,can be more effective accumulated at the tumor site.The robust tumor killing effect and little side effect was observed.3.The conductive mussel shell-derived myocardial patch,exhibited with good shape,uniform porous and rich pores.Myocardial cells can adhere and grow on the surface,and extend well to form a crossing net between cells.The Functional syncytium contract synchronyly with higher expression of CX43 and alpha-actinin proteins.The conductive mussel shell-derived myocardial patch can maintain myocardial cell retention and survival in the infarct area and promoting them migration to the host site of infarction.The high level of vascularization,the increase of the thickness of the ventricular wall,the narrowing of the infarct size and the low degree of fibrosis was observed.The structure and function of infarcted heart was all be promoted.ConclusionIn vitro and in vivo experimental indicated that mussel adhesion protein and mussel shell derived biomaterials has a good application prospect.