Study Of Single Nucleotide Polymorphisms In Vitamin D Receptor Gene Polymorphisms And Permanent Tooth Caries Susceptibility In Adolescent

OBJECTIVES:Dental caries is a multifactorial infectious disease.In this study,we investigated whether vitamin D receptor(VDR)gene single nucleotide polymorphisms(SNPs)were associated with permanent tooth caries susceptibility in Chinese adolescents.METHODS:A total of 200 dental caries patients and 200 healthy controls aged 12 years were genotyped for VDR gene polymorphisms using the PCR-restriction fragment length polymorphism(PCR-RFLP)assay.All of them were examined for their oral and dental status with the WHO criteria,and clinical information such as bleeding on probing(BOP),Volpe Manhold(VM-value),the Decayed Missing Filled Teeth Index(DMFT)were evaluated.Genomic DNA was extracted from the buccal epithelial cells.The four polymorphic SNPs(Bsm1,Taq1 Apa1 and Fok1)in VDR were assessed for both genotypic and phenotypic susceptibilities.RESULTS:Among the four examined VDR gene polymorphisms,the increased frequency of the CT and CC genotype of the FokI VDR gene polymorphism was associated with dental caries in 12-year-old adolescent,compared with the controls(X2=17.8135 p=0.000).Moreover,FokI polymorphic allele C frequency was significantly increased in the dental caries cases,compared to the controls(X2 =14.144,p=0.000,OR=1.730,95%CI=1.299-2.303).However,the other three VDR gene polymorphisms(BsmI,TaqI and ApaI)showed no statistically significant differences in the caries groups compared with the controls.CONCLUSIONS:VDR-FokI gene polymorphisms may be associated with susceptibility to permanent tooth caries in Chinese adolescent.