The Study Of The Correlation Between The Immune-Inflammatory Cytokines And The Characteristics Of "Toxin" In Chronic Heart Failure

Objective:Using a combine method of theoretical research,clinical research and experimental research,the Study of the Correlation between the immune-inflammatory cytokines and the Characteristics of "Toxin" in Chronic Heart Failure.In theoretical research,the conception,characteristics and meaning of "Toxin" were been summarized.The theory of "Toxin"causing the cardiovascular disease were been summarized.Homogeneous features and Basic pathological changes were compared between "Toxin"and chronic heart failure.In theory,we proposed the evolutionary process of "Toxin" in chronic heart failure,which could provide theoretical foundation for the theory of "Toxin" in chronic heart failure.In clinical research,the case control study was designed in order to compare difference on the expression of immune-inflammatory cytokines between health and chronic heart failure.At the same time,clinical symptoms and pattern factors in traditional chinese medicine(TCM)were been compared chronic heart failure between with "Toxin" and without"Toxin",which may provide clinical evidence to support the theory of"Toxin" causing chronic heart failure.In experimental research,using the treatment method of tonifying Qi,activating blood and detoxication on pressure overload in rat induced by abdominal aortic constriction,the heart function,the expression of BNP and NT-pro BNP,T cell immune function,and the expression of inflammatory cytokines,such as TNF-alpha、IL-6 and IL-1beta induced by TLR4 pathway were studied in order to disproof the "Toxin" existence in chronic heart farilure.Method:Data sources included CNKI,Wan Fang database,Chongqing VIP,chinese biomedicine literature database through december 30,2014.The key words were "Toxin","characteristics of toxin","meaning of toxin".All the literature about "Toxin" and "Toxin" conception were gathered."Toxin"were taken place in cardiovascular disease,including coronary heart disease,hypertension,hyperlipidemia,diabetes mellitus with coronary heart disease were summarized.Based on all the literature,we compared the characteristic between chronic heart failure and "Toxin".In addition,"heart failure"," TCM etiopathogenisis and pathogenesis" and/or"patterns" and/or "patterns cells" were as key words.the procedure of pathological developing in chronic heart failure and "Toxin" were compared.Based on the literature research,experts discussion were carried out and make up the form for distinguishing whether the chronic heart failure patients with or without "Toxin".we use case control study to in survey and analyze the relationship of immune-inflammatory expression and chronic heart failure.According to matched case-control(be in the ratio of 1:1:1:1:1)study,we chosen 40 healthy people from health examination center of the First Affiliated Hospital of Guangdong University of Chinese Medicine and 40 NYHA I class patients,40 NYHA II class patients,40 NYHA III class patients and 40 NYHA IV class patients from cardiovascular department of the First Affiliated Hospital of Guangdong University of Chinese Medicine.Multiple cytokines technology of Luminex were adapted to test the expression of TNF-α、IL-1β、IL-6、IL-8、CD14.Enzyme-linked immunosorbent assay(ELISA)were used to test the expression of TLR4 and IL-18.Clinical physician distinguished the chronic heart failure with "Toxin" and without "Toxin".The correlationship between two of them were surveyed and analyzed in TNF-αα,IL-1β,IL-6,IL-8,IL-18,CD14,TLR4,the patterns cells of disease location,the patterns cells of pathogenesis and symptoms.R project software(3.1.3 version)were used.Quantitative data were descript mean values ± standard deviation for normal distribution,while non-normal distribution were descript by median.The data to compare different groups were analyzed by using student’s test and one-factor analysis of variance for normal distribution continuous variable,and wilcoxon rank sum test and Kruskal-Wallis test for non-normal distribution continuous variable.About binary variable or categorical variable were analyzed by chi-square test and Fisher test.Models of rats with pressure overload induced by abdominal aortic constriction.70 male SD rats were randomly allocated to sham group,HF(model of heart failure),AHigh(A prescription with high dosage group),AMedium(A prescription with medium dosage group),ALow(A prescription with low dosage group),Bdrug((A prescription group)and WM(western medicine).Pressure overload model of rats induced by abdominal aortic constriction.treatment duration was 24 weeks.After treatment,Left Ventricular Ejection Fraction(LVEF%)and left ventricular fractional shortening(LVFS%)were observed.BNP and NT-pro BNP were tested.TNF TNF-alpha、IL-6、IL-lbeta were tested by ELISA.Th1、Th2、Th17 and Treg were tested by flow cytometry test.The expression of TLR4,MyD88 and NFκ B in heart and spleen were test by quantitative real-time polymerase chain reaction(Q-RT-PCR).one-factor analysis of variance and Bonferroni were adapted.Homogeneity test of variances were test by bartlett.test.Least Significant Difference test and Tukey’s range test were used to in multiple comparisons.Result:Literature research,summary "toxic" evil definition,connotation and characteristics,summarizes the common basic diseases,chronic heart failure,hypertension,coronary heart disease and viral myocarditis disease and "poison" pathogenic closely related.From the characteristics of the disease and contrast "poison" pathogenic and chronic heart failure(CHF)pathogenic characteristics,analysis results show "toxic" evil pathogenic is violent,rapid onset,rapid change,complex,intractable,illness lingering heals hard,and easy to dry injury Yin,corrupt body hylomorphism,structure and function of viscera damage,to cause serious harm to human body,and chronic heart failure(CHF)in the pathogenesis of many evil with stubbornness;disease complex,involving more dirty,easy to change,life-threatening characteristics with consistency.Based on the common basis of diseases in chronic heart failure "toxic" evil causes and combination of ancient and modern on chronic heart failure due to the pathogenesis occurs development process,the chronic heart failure"poison" evil formation reason is based on chronic gas,blood,yin and Yang Xushuai,resulting in blood stasis,phlegm,and water to drink,the real evil,mutual stalemate,continue to accumulate,and qualitative change,the formation of "toxic" evil,resulting in target organs etc.damage.Based on "toxic" evil’s strong attachment,and the original real evil still exists in vivo,Gu often appear,phlegm and toxin and stasis toxic,poisonous water and heat toxin and see,creating a vicious cycle,resulting in complex disease,refractory lingering illness and retains part of the original basic pathogenesis characteristics,but they are different on some basic pathogenesis.The study provided a theoretical basis for the pathogenic sick leave of chronic heart failure..Clinical case-control study,221 patients with chronic heart failure,40 healthy people.Chronic heart failure patients with the combination of hypertension accounted for 141 people,accounting for 63.80%,coronary heart disease,96 people,accounting for 43.43%,combined hyperlipidemia 47 people,accounted for 21.27%,with arrhythmia 25 people,occupied 11.31%,rheumatic heart disease 23,10.41%accounted for,diabetic heart disease 15 people,6.79%accounted for,with pulmonary heart disease 10 people,accounted for 4.52%,merger and expansion cardiomyopathy 8 people,3.61%.Compared with healthy people,the expression of IL18,IL-1,IL-8,IL-6 and TNF-in patients with chronic heart failure was statistically significant,P<0.05.According to NYHA classification,with increasing heart failure NYHA functional classification,serum TNF alpha,IL-6,IL-lbeta,IL-8 and IL-18 gradually increased significantly,with statistical significance,P<0.05;according to the left ventricular ejection fraction,left ventricular ejection fraction greater than 50%of chronic heart failure patients,the serum levels of TNF-alpha,IL-1,IL-6,IL-8 and IL-18 expression level was significantly higher than that of left ventricular ejection fraction less than 50%of patients with chronic heart failure(CHF,with statistical significance.According to the frequency of heart rate,heart rate greater than 100 beats/min with TNF alpha,IL-1lbeta,IL-6,IL-8 and IL-18 expression level is higher than that of chronic heart failure heart rate of less than 100 beats/min patients,the difference was statistically significant,P<0.05.The clinical study,investigation of 221 patients with chronic heart failure,disease involving the heart of 203 people,accounting for 91.86%;disease involving the spleen were 101 people,accounting for 45.70%;disease involved in the lung in 62 people,28.05%;disease involving the kidney 42 people,accounting for 19.00%,disease involving the liver 27 people(12.22%.With the increase of the number of TCM syndromes in chronic heart failure patients,IL-lbeta,IL-6,TNF-alpha,IL-8 and IL-18 were significantly increased,and were statistically significant,P<0.05.The relationship between the number of the disease and the number of the disease and the occurrence of the pathogen was closely related to the occurrence of the disease,and it was statistically significant,P<0.05.The presence of chronic heart failure "poison" evil and chronic heart failure(CHF)in relation to the overall disease syndrome prime number,virtual STD syndrome of prime number and the real evil disease syndrome prime number.There is an association between,the difference was statistically significant.And chronic heart failure without "toxic" evil exists compared with patients and chronic heart failure "poison" evil is involved in patients with a high proportion of spleen,lung,kidney and liver,and the difference is statistically significant.P<0.05;chronic heart failure "poison" evil exists in patients with and without "poison"evil patients involved in the proportion of heart are very high,difference of no statistical significance.In disease of syndrome factors of chronic heart failure "poison" evil are involved in patients with Yin,Yang and phlegm,stop water ratio high,and the difference isstatistically significant,P<0.05;chronic heart failure "poison" evil exists in patients with and without "poison" evil exists in patients with Qi deficiency are very high,no statistically significant difference,P>0.05;chronic heart failure "poison" evil exists in patients with and without poison "evil exists in patients with Qi deficiency were lower,the difference was not statistically significant,P>0.05.In chronic heart failure clinical symptoms and pathogenic toxin showed in shortness of breath,dyspnea,edema of lower extremities,cough,expectoration,ascites,neural fatigue,weakness of limbs,chest tightness,facial edema,spontaneous sweating,night sweats,stomach cold limbs cold symptoms appear proportion,two groups are different,with statistical significance,P<0.05.Among them,chronic heart failure "poison" evil exists in patients with,difficulty in breathing,lower extremity edema,cough,expectoration,ascites,neural fatigue,weakness of limbs,chest tightness,facial edema,spontaneous sweating,night sweats,stomach cold limbs cold ratio of symptoms,over a "poison" evil exists in patients with chronic heart failure,with statistical significanc,P<0.05e;"poison" evil exists in patients with higher proportion of shortness of breath for patients with chronic heart failure without "poison" evil exists,the difference is statistically significant,P<0.05.Animal experiments showed that in influence on pressure load in rats with chronic heart failure cardiac function,compared with the sham operated rats,LVEF%of pressure load models of heart failure group decreased significantly;compared with the model of heart failure,a medicine high dose group,a drug dose group,drug a low dose group,B medicine group and Western medicine group rats LVEF%were improved,which a drug high dose group increased statistically significant,P<0.05;compared with the western medicine group,a drug high dose and B were increased,a drug high dose increased with statistical significance,;compared with B drug,a drug high dose increase was statistically significant.Compared with the sham operation group rats,the pressure load models of heart failure group LVFS%decreased significantly,was statistically significant,P<0.05;compared with the CHF model,drug a high dose group,a drug dose group,drug a low dose group,B medicine group and Western medicine group rats LVEF%were improved,which a drug high dose group increased with statistical significance,P<0.05;compared with the western medicine group,a drug high dose and B were increased,a drug high dose increased with statistical significance,P<0.05;compared with B drug,a drug high dose increased with statistical significance.Compared BNP and BNP administration significantly increased,and the influence of the BNP and NT Pro BNP expression in sham operation group rats compared with pressure overload chronic heart failure model in rats plasma BNP,there is statistical significance,P<0.05;and heart failure models are compared,and a medicine high dose group,a drug dose group,a lower dose group,B group,western medicine group rats were decreased with statistical significance,P<0.05;and the western medicine group compared to a drug high,medium and low dose B expression were increased,there was no statistical significance,P<0.05,and B drug,a drug high dose BNP expression decreased,and no statistical significance;a drug high,medium and low dose,the expression of BNP delivery times increased.There was no statistical significance,P<0.05.Compared with the sham operation group rats,plasma NT-proBNP pressure overload rat model of chronic heart failure increased significantly,there was statistical significance,P<0.05,compared with the model of heart failure;A,high dosage group,middle dose group,A drug drug A low dose group,western medicine group,B treatment group rats NT-pro BNP were decreased,with statistical significance,P<0.05;compared with the western medicine group,high dosage of A,NT-pro increased the expression of BNP,no statistical significance,P>0.05,compared with B A;medicine,medicine high dose NT-pro BNP expression decreased,with statistical significance,P<0.05,B;compared with the drug,reduce the dose of NT-pro BNP expression of A in medicine,no statistical significance,P>0.05,compared with the B drug;A drug,low dose NT-pro BNP expression increased,with statistical significance,P<0.05.Compared with the high dose of A,the expression of BNP NT-pro in A was increased,and it was statistically significant,P<0.05.In effect on pressure overload in chronic heart failure rat TNF alpha,IL-6,IL-1 beta,compared with the sham operation group,pressure load in chronic heart failure rat TNF-expression decreased significantly,was statistically significant,P<0.05;compared with the rat model of pressure overload in chronic heart failure group,a medicine high dose group and Western Medicine group TNF alpha significantly reduced,was statistically significant,P<0.05;compared with the rat model of pressure overload in chronic heart failure group,a drug,low dose group and B to TNF alpha group decreased expression,no statistically significant,P>0.05;compared with the western medicine group,a drug in the high dose group increased,but no statistical significance,P>0.05.(2)and sham operation group compared pressure load in chronic heart failure rat IL-6 expression was significantly decreased,was statistically significant,P<0.05;compared with the rat model of pressure overload in chronic heart failure group,a medicine high dose group and Western medicine group IL-6 was significantly decreased,P<0.05;compared with the rat model of pressure overload in chronic heart failure group,reduce drug a high dose group,drug a low dose group and B group IL-6,no statistically significant;compared with the western medicine group,a drug in the high dose group increased,but no statistical significance,P>0.05.(3)compared with the sham operation group,pressure load in chronic heart failure rat IL-1 expression decreased significantly,was statistically significant,P<0.05;a medicine high dose group and Western medicine group IL-1 beta compared with chronic heart failure model of pressure overload rats were significantly reduced,P<0.05;compared with the rat model of pressure overload in chronic heart failure group,reduce drug a high dose group,drug a low dose group and B group IL-1 beta,no statistically significant,P>0.05;compared with the western medicine group,a drug in the high dose group increased,but is not statistically significant,P>0.05.In effect on chronic heart failure model of pressure overload rat T cell immunity,(1)compared with sham operation group,Thl%pressure load model of chronic heart failure rats decreased expression,no statistically significant,P>0.05;compared with the rat model of pressure overload in chronic heart failure group and drug a low dose group,B medicine group and Western medicine group Thl%expression significantly decreased with statistical significance,P<0.05;compared with the rat model of pressure overload in chronic heart failure group and decreased expression had a medicine high dose group and middle dose group of Thl%,but without statistical significance,P>0.05.Th2%expression Th2%expression Th2%reduced Th2%low dose compared with expression of Th2%expression Th2%rats(2)and sham operation group,the pressure load in chronic heart failure model group rats decreased with statistical significance,P<0.05;and pressure load in chronic heart failure model rats compared group,a high dose group and Western medicine group increased,with statistical significance,P<0.05,and the western medicine group and a drug group and B group,there is significant difference,P<0.05;and B medicine is a medicine high dose group increased,there was no statistical significance,and B drug is a drug dose group decreased and there was no statistical significance,P>0.05,and B drug,a drug of low dose group expression significantly decreased,there was significant,P<0.05.(3)compared with the sham operation group,pressure load model of chronic heart failure rats rats Thl%/Th2%expression decreased,but without statistical significance.P>0.05;compared with the pressure load model of chronic heart failure rats rats,a medicine high dose group,drug a low dose group,drug a low dose group,B medicine group and Western medicine group,the Thl%/Th2%ratio,followed a rising trend,but no statistical significance,P>0.05.(4)and sham operation group compared pressure load in chronic heart failure rat model group the percentage of Th17 was decreased expression,but no statistical significance,P>0.05;compared with the rat model of pressure overload in chronic heart failure group,a medicine high dose group,a drug dose group and Western medicine group,the percentage of Th17 was expression increased,but there was no statistical significant.;compared with the rat model of pressure overload in chronic heart failure group and drug a low dose and B group Th1%decreased expression,not with statistical significance,P>0.05.(5)compared with the sham operation group,chronic pressure overload heart failure model rats group Treg%decreased,with statistical significance,P<0.05,compared with pressure overload;chronic heart failure rat model group,A high dosage group and Western medicine group the expression of Treg%was increased,with statistical significance,P<0.05,compared with pressure overload;chronic heart failure model rats,increased dose group and B treatment group the expression of Treg%A in medicine,was not statistically significant,P>0.05,compared with pressure overload;chronic heart failure rat model group,A low dose group decreased Treg%expression,but not statistically significant,P>0.05;compared with the western medicine group,the expression of A in high dosage group Treg%is a bit low,but no statistical significance,P>0.05;compared with the western medicine group,drug dose of A,reduce the expression of A in low dose group and drug treatment group B Treg%,with statistical significance,P<0.05;compared with B group,the expression of A in high dosage group of Treg%was significantly decreased,with statistical significance,P<0.05.(6)compared with the sham operation group,pressure load in chronic heart failure rat model group Th17%/Treg%increased the expression of,with statistically significant,P<0.05;compared with the rat model of pressure overload in chronic heart failure group,a medicine high dose group and Western medicine group Thl7%/Treg%expression were decreased,and the western medicine group than in the traditional Chinese medicine group decreased significantly,but does not have statistical significance.P>0.05;compared with the rat model of pressure overload in chronic heart failure group and a drug in the dose group,a drug of low dose group and B group of Th17%/Treg%expression increased,not with statistical significance,P>0.05.Animal experiments show that the expression of cardiac TLR4 pathway in rats with chronic heart failure induced by pressure load is affected by the expression of cardiac pathway.(1)Compared with control group,pressure load TLR4 expression in rats with chronic heart failure model group significantly increased,statistically significant,P<0.05;And pressure load in rats with chronic heart failure model group compared with A high dose group and western medicine group of TLR4 expression,statistically significant,P<0.05;Compared with pressure load of chronic heart failure model rats group,A drug dose group,low dose group and B group of TLR4 expression,not statistically significant,P>0.05;Compared with the western medicine group,A drug high TLR4 expression of the high dose group,but no statistical significance;Compared with the western medicine group,high dose,low dose group and B increased the expression of TLR4 medicine group,was statistically significant P<0.05;Compared with group B drug,A drug and the expression of TLR4 high dose group was obviously higher than that of group B drug,statistically significant,P<0.05;Compared with group B drug,A drug dose group of the expression of TLR4 is reduced,without statistical significance,P>0.05;A drug high,medium and low three doses,A drug high dose group of TLR4 expression decreased obviously,and A drug dose group and A drug,low dose group,statistically significant,P<0.05.(2)compared with control group,chronic heart failure pressure load model rats group considerably increase the MyD88 expression,statistically significant,P<0.05;And pressure load in rats with chronic heart failure model group,compared to A medicine MyD88 express high dose group and western medicine group were significant,statistically significant,P<0.05;Compared with pressure load of chronic heart failure model rats group,A drug dose group,low dose group and B group of MyD88 expression,not statistically significant,P>0.05;Compared with the western medicine group,A drug high MyD88 expression of the high dose group,but no statistical significance;Compared with the western medicine group,A drug dose,low dose group and B group in the expression of MyD88,was statistically significant,P<0.05;Compared with group B drug,A drug TLR4 expression of the high dose group was obviously reduced,statistically significant,P<0.05;Compared with group B drug,A drug dose and low dose group of the expression of TLR4 rise obviously,statistically significant,P<0.05;A drug high,medium and low three doses,A medicine MyD88 express high dose group decreased obviously,and A drug dose group and A drug,low dose group,statistically significant,P<0.05.(3)compared with control group,chronic heart failure pressure load model rats group considerably increase the nf-kappa B expression,statistically significant,P<0.05;And pressure load in rats with chronic heart failure model group,compared to A drug high dose group and western medicine group nf-kappa B expression were significantly,statistically significant,P<0.05;Compared with pressure load of chronic heart failure model rats group,A drug dose group,low dose group and B group nf-kappa B expression,not statistically significant,P>0.05;Compared with the western medicine group,A drug high nf-kappa B expression of the high dose group,but no statistical significance,P>0.05;Compared with the western medicine group,A drug dose,the expression of A medicine low-dose nf-kappa B,statistically significant,P<0.05;Compared with group B drug,A drug the nf-kappa B expression of the high dose group was obviously lower than that,statistically significant;Compared with group B drug,A drug dose group of the nf-kappa B increased,no statistical significance,P>0.05;Compared with B medicine A nf-kappa B is reduced,low dose group had no statistical significance,P<0.05;A medicine high,medium and low three doses,A medicine high dose group of the nf-kappa B expression decreased obviously,and A drug dose group and A low dose group,with statistical significance,P<0.05(4)as shown in table 3-10,P38MAPK expression level of qRT-PCR,compared with the sham operation group,the expression of rat model of chronic heart failure group P38MAPK pressure load increased significantly,with statistical significance,P<0.05,compared with pressure overload;chronic heart failure rat model group,A high dose,middle dose group,B treatment group,western medicine group the expression of P38MAPK was significant,with statistical significance,P<0.05,compared with pressure overload;chronic heart failure rat model group,A low dose group P38MAPK expression significantly,was not statistically significant,P>0.05;compared with the western medicine group,high dosage group,A,A drug in the dose group,A low dose group and B treatment group the increased expression of P38MAPK,was not statistically significan,P>0.05t,compared with B;drug,dosage of A in high dosage and A medicine P38MAPK expression decreased,but not statistically significant,P>0.05,compared with B A;drug drug,low dose of P38MAPK expression increased,but not statistically significant,P>0.05.(5)compared with the sham operation group,the expression of rat model of chronic heart failure group JNK pressure load increased significantly,with statistical significance,P<0.05,compared with pressure overload;chronic heart failure rat model group,A high,medium and low dose group,B treatment group and Western medicine group,the expression of JNK was significantly decreased,with statistical significance,P<0.05;compared with the western medicine group,higher dose and B medicine group the expression of JNK A in high dosage group,A,was not statistically significant P>0.05;compared with the western medicine group,low dose group,A JNK expression increased,with statistical significance,P<0.05,compared with B A;medicine,medicine high dose group the decreased expression of JNK,not statistically significant,P>0.05,compared with the B A;medicine,drug dose and A low dose of the drug increased the expression of JNK,but not statistically,P>0.05.Animal experiments showed that,the expression of TLR4 pathway in chronic heart failure group spleen rat model of pressure overload,(1)the expression of splenic TLR4 pathway,compared with sham operation group,the expression of rat model of chronic heart failure group TLR4 pressure load increased significantly,with statistical significance,P<0.05,compared with pressure overload;chronic heart failure rat model group,A in high dosage group and Western medicine group TLR4 expression were significant,with statistical significance,P<0.05,,compared with pressure overload;chronic heart failure rat model group,A drug in the dose group,low dose drug A group and B treatment group decreased TLR4 expression,was not statistically significant,P>0.05;compared with the western medicine group,the expression of A in high the high dose of TLR4 group,but no statistical significance,P>0.05.Animal experiments showed that,the expression of TLR4 pathway in chronic heart failure group spleen rat model of pressure overload,(1)the expression of splenic TLR4 pathway,compared with sham operation.group,the expression of rat model of chronic heart failure group TLR4 pressure load increased significantly,with statistical significance,P<0.05,compared with pressure overload;chronic heart failure rat model group,A in high dosage group and Western medicine group TLR4 expression were significant,with statistical significance,P<0.05,compared with pressure overload;chronic heart failure rat model group,A drug in the dose group,low dose drug A group and B treatment group decreased TLR4 expression,was not statistically significant,P>0.05;compared with the western medicine group,the expression of A in high the high dose of TLR4 group,but no statistical significance,;compared with B group,the expression of A in high dosage group of TLR4 was significantly higher than that in B group,no statistical significance,A;medicine high,low dose group,in three,the expression of TLR4 was gradually increased,but the difference was not statistically significant,P>0.05.(2)spleen MyD88 pathway expression compared with the sham operation group,the expression of rat model of chronic heart failure group MyD88 pressure load increased significantly,with statistical significance,P<0.05,compared with pressure overload;chronic heart failure rat model group,A high dosage group and Western medicine group MyD88 expression were significant,with statistical significance,P<0.05,compared with the pressure;the load model of chronic heart failure rats,A drug in the dose group,low dose drug A group and B treatment group decreased MyD88 expression,was not statistically significant,P>0.05;compared with the western medicine group,high dosage group,the expression of A MyD88 is high,but the difference was not statistically significant,P>0.05;compared with B group,the expression of A medicine the middle dose group and low dose group A drug MyD88 gradually increased,no statistically significant,P>0.05,A;high,medium,low drug dose three,MyD88 expression increased,with statistical significance P<0.05,.(3)splenic NF-kappa B expression level of qRT-PCR,compared with the sham operation group,group NF-kappa B expression in rat model of chronic heart failure load pressure increased significantly,with statistical significance,P<0.05,compared with pressure overload;chronic heart failure rat model group,A high dosage group and Western medicine group NF-kappa B expression significantly,with statistical significance,P<0.05,compared with pressure overload;chronic heart failure rat model group,A drug in the dose group,low dose A medicine group and B treatment group of NF-kappa B expression decreased,was not statistically significant;compared with the western medicine group,high dosage group,the expression of A NF-kappa B low,but no statistical meaning,;compared with B group,the expression of A in high dosage group of NF-kappa B low,with statistical significance,P<0.05 A;comparative medicine high,medium,low dose three,A in high dosage group of NF-kappa B expression was significantly decreased,compared with dose group and low dose group A drug A drug,with statistical significance,P<0.05.(4)the expression level of qRT-PCR P38MAPK in spleen,compared with sham operation group,the expression of rat model of chronic heart failure group P38MAPK pressure load increased significantly,but not statistically significant,P>0.05,compared with pressure overload;chronic heart failure rat model group,A high dose,middle dose group,B treatment group and Western medicine group,the expression of P38MAPK all decreased significantly,with statistical significance,P<0.05,compared with pressure overload;chronic heart failure rat model group,A low dose group the expression of P38MAPK was significantly decreased,no statistically significant,P>0.05;compared with the western medicine group,high dose group and B treatment group the expression of P38MAPK A in high dosage group,A,no statistical significance,;compared with the western medicine group,A low dose drug group increased,with statistical significance,P>0.05,compared with B;drug,dosage of A in high dosage and drug A in P38MAPK was decreased,but the difference was not statistically significant,P>0.05,compared with B A;drug drug,low dose of P38MAPK expression increased,but the difference was not statistically significant,P>0.05.(5)the expression level of qRT-PCR JNK in spleen,compared with sham operation group,group JNK expression in rat model of chronic heart failure significantly increased pressure load,was not statistically significant,P>0.05,compared with pressure overload;chronic heart failure rat model group,A high,medium and low d...