Study On The Roles Of MiR-124 And USP39 In The Regulation Of Glioma Development

Glioma is a common malignant primary tumor with high incidence and high mortality.Despite the advanced treatment methods such as surgery,radiotherapy,chemotherapy and imaging diagnostic technology are improving,the life expectancy of patients with gliomas is only 14 months,and the survival time of patients with malignant gliomas is not significantly improved.It is of great scientific significance and clinical significance to explore the mechanism of glioma development and find new ideas and new targets for the treatment of glioma.MicroRNA has been proved to play an important role in the development of tumor.A variety of microRNA are abnormal expressed in glioma and microRNA could affect the proliferation,migration and invasion of glioma cells.We used the glioma cell line U87 to study the effect of miRNA-124 on the development of glioma.It is found that the expression of miR-124 in glioma samples and cell lines is significantly down regulated.miR-124 could inhibit the growth,migration and invasion of U87 glioma cells,and the mechanism involves miR-124 inhibiting the expression of target gene Vimentin.At the same time,miR-124 could inhibit the growth of glioma in nude mice.The study revealed that the roles of miR-124 in glioma and its potential application as a potential therapeutic target for glioma.Deubiquitinating enzyme is an important effector of ubiquitin proteasome system,and it has many biological functions.It is becoming a new target for anticancer drugs.USP39 has the domain of the ubiquitination enzyme,which belongs to the member of the family of the ubiquitination enzyme family.USP39 plays an important role in the assembly process of mRNA mature splicing.It is also found that USP39 is associated with some tumorigenesis,but the role of USP39 in the genesis and development of glioma still not known.By bioinformatic analysis and histological detection,we found that high expression of USP39 in glioma;using U87 and U251 glioma cell lines,siRNA interference or lentivirus vector of USP39 interference,the data showed that USP39 could promote glioma proliferation,migration and invasion.And USP39 interference could inhibit the expression of ADAM9 mRNA and promote the expression of PTEN mRNA.This study extends the understanding of USP39 function,and the results will provide new ideas and new targets for the prevention and treatment of glioma.