| Objective To investigate the effects of vasoactive intestinal peptide(VIP)on the proliferation,angiogenesis and epithelial mesenchymal transition in prostate cancer PC-3and DU145,through the experimental method of cell culture and animal experiments,by using CCK-8,PCR,Westernblot,Transwell cell technology.Methods Treatment of PC-3 and DU145 prostate cancer cells with the concentration of 0,25,50 and 100 VIP,respectively.The changes of cell proliferation ability of PC-3 cells were detected by CCK-8 method.in 24,48 and 72 h,the expression of Cyclin D1,bcl-2,MMP-9m RNA and protein in PC-3 cells was detected with PCR and Westernblot.Construction of PC-3 model of nude mice treated by 100nmol/l VIP,measuring the volume of transplanted tumor and the changes of m RNA VEGF and microvessel density.The scratch test and Transwell assay was performed to detect DU145 cell motility,migration and invasion ability changes,the changes of E-cadherin,Vimentin,m RNA Snail and protein in DU145 cells were detected by PCR and Westernblot.Results With the increase of the concentration of VIP and time,the proliferation of PC-3cells gradually becomes strong,the expressions of Cyclin D1,bcl-2,MMP-9 m RNA and protein were increased,the difference is statistically significant(P<0.05).In nude mice after100nmol/l VIP treatment,nude mice transplantation tumor volume,content of VEGFm RNA and MVD is greater than control group,the difference is statistically significant(P<0.05).With the increase of the concentration of VIP,row mark experiment indicates a increasing exercise capacity in DU145,transwell migration and invasion experiments showed that the number of the cells crossing through the cell number was increasing,the expressions of E-cadherin m RNA and protein was decreasing,Vimentin and Snail m RNA and protein expression was increasing,the difference is statistically significant(P<0.05).Conclusion VIP promotes the proliferation of hormone independent prostate cancer cells by Cyclin D1 and Bcl-2,by stimulating the proliferation of blood vessels.It also promotes ability of diffusion and metastasis and metastasis.androgen independent prostate cancercell by promoting the degradation of extracellular matrix and epithelial-mesenchymal transition. |
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