The Clinical Significance Of CDK1 Expression And The Expression Trend Of The Related MiR-192 In Oral Squamous Cell Carcinoma

Purpose:To determine the clinical significance of cyclin-dependent kinase 1(CDK1)in 77 OSCCs using immunohistochemical methods and the expression level of the related miR-192 in 8 oral squamous cell carcinomas(OSCC)by using qPCR.Method:Immunohistochemical expression of CDK1 was compared with various clinicopathological features in 77 OSCC and 60 controlled epithelia adjacent to the tumours.In addition,correlation of CDK1 expression and prognostic and the 5-year accumulative survival rate,local recurrence,lymph node metastasis of OSCC were investigated.A total of 8 cases of OSCC with healthy oral tissues were selected.These samples were detected by using qPCR,and the express trend features of OSCC were analyzed.Results:The CDK1 protein was expressed in 52 cases of 77 tumor tissues(67.5%),compared with 21 cases of 60 controlled(35.0%).The expression of CDK1 was significantly correlated with the histological grade of OSCC(P<0.05).The CDK1 protein was over-expressed in recurrent tumors or in those with lymph node metastasis.Statistical analysis showed a significant reduction in the 5-year accumulative survival rate in CDK1 positive cases compared with CDK1 negative cases(P<0.05).Namely,the CDK1 positive patients had poor prognosis.Both the expression of miR-192 and pri-miR-192 was highly expressed in OSCC,especially for miR-192(P=0.000516<0.01).Conclusions:The expression of CDK1 might serve as malignant degree and prognostic markers for the survival of OSCC.Over-expression of miR-192 was found in OSCC,compared with healthy oral tissues.Overexpression of miR-192 might have better predictive value in the prognosis of oral squamous cell carcinoma together with CDK1 which deserves further study.