The Expression And Significance Of Leptin And Adiponectin In Nonalcoholic Fatty Liver Disease Patients

AIM To detect level of leptin in serum and the expression of leptin,OB-R and PI3-K/Akt kinase pathway in nonalcoholic fatty liver disease patients.Materials and Methods RIA was used to detect the level of serum Letpin in 30 patients with nonalcoholic fatty liver disease patients and 30 controls, and to analyze the clinical indexes of fasting blood glucose, total cholesterol, glycerin three fat, C-peptide, insulin, body mass index, and the relationship between Letpin and insulin resistance, blood lipids and non alcoholic fatty liver disease.The expression of leptin,OB-R and PI3-K/Akt kinase pathway were examined by means of immunohistochemistry. The level of leptin in serum was measured by RIA. The expression of adiponectin in 30 patients with nonalcoholic fatty liver disease patients and normal control group was detected by immunohistochemical method. (RIA) was determined by radioimmunoassay in 33 cases of nonalcoholic fatty liver disease patients and 30 cases of control group, leptin, hyaluronic acid (HA), laminin (LN), type III collagen peptide (PC III) and type IV collagen (IV-C).Results Letpin, BMI, fasting insulin and insulin resistance index (IR HOMA) in nonalcoholic fatty liver disease patients patients were significantly higher than those in the control group (<0.05 P). Male and female Letpin levels were higher than male and female control group (<0.05 P). The HOMA IR is the dependent variable, LETPIN, BMI, bloodsugar level, total cholesterol and triglyceride as independent variables, the multivariate stepwise regression analysis into.The level of leptin in serum of the nonalcoholic fatty liver disease patients is higher than that of controis.Leptin,OB-R and PI3-K(p85) were expressed higher in nonalcoholic fatty liver disease patients than in the controls. Phospho-Akt kinase was expressed lower in nonalcoholic fatty liver disease patientsthan in the controls. There was a significant positive correlation between leptin in serum and the optical density value of leptin in nonalcoholic fatty liver disease patients(r= 0.413, P< 0.05),and the optical density value of leptin and PI3-K(p85) also had a positive correlation(r= 0.365, P< 0.05). There was a significant negative correlation between the gray values of PI3-K(p85) and phospho-Akt kinase (r=-0.854, P< 0.05). In agreement with significantly lowered serum adiponectin level in nonalcoholic fatty liver disease patients patients(P < 0.05),expression of adiponectin was significant lowered in nonalcoholic fatty liver disease patients than in control tissue (all P< 0.05). There were no significant differences in serum PC,-C, LN, HA and P in mild nonalcoholic fatty liver disease patients patients, and in patients with moderate and severe nonalcoholic fatty liver disease patients were significantly higher than those in control group (P< 0.05), and HA was the most significant (P< 0.01). The serum leptin level was not correlated with hepatic fibrosis index (P> 0.05).Conclusions Leptin and adiponectin plays a key role in the occurrence and development of nonalcoholic fatty liver disease patients. Leptin may be involved in nonalcoholic fatty liver disease patients pathogenesis by activating PI3-K/Akt kinase pathway via OB-R and the defective leptin activation of PI3-K is a novel mechanism of leptin resistance in nonalcoholic fatty liver disease patients. Leptin is closely related to the process of liver fibrosis in patients with nonalcoholic fatty liver disease.