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The Research On Design And Application Of SPR Array Imaging Sensor

Posted on:2015-02-17Degree:DoctorType:Dissertation
Country:ChinaCandidate:Z H HuangFull Text:PDF
GTID:1268330428984574Subject:Measuring and Testing Technology and Instruments
Abstract/Summary:PDF Full Text Request
Surface Plasmon Resonance (SPR) bio-sensors have been widely applied in the areas of environment monitoring and food safety for its real-time, label-free measurement and high sensitivity. The need of high-througput SPR system is rising. Array SPR imaging sensor is key to perform SPR highthrougput detection. The aim of the project is to build an array SPRi sensor. In this assay, an array SPR imaging sensor has been built and tested,including:1. Based on SPR gold film polarization control method, a contrast-enhancing prism polarization control method is proposed. A theoretical model was established and the theory was proved by experiment.2. The primary optical parameter have been analyzed and optimized. The array SPRi sensor has been designed. The linear light contains both transverse-electric (TE) and transverse-magnetic (TM) waves. The polarization of the reflected light is modulated by1/4waveplate and analyzer. The background signal is suppressed to get a high contrast imaging.3. Combined methods to improve the performance of the surface plasmon resonance array sensor are proposed. A self-reference method is adpoted to suppress the baseline drift. The Hilbert-Huang transform-based signal post-processing algorithm is employed to reduce high-frequency noise. An adaptive moving-average method was used to reduce noise while preserving important high-frequency signal characteristics. The combined methods improve the baseline drift by an order of magnitude and achieve a higher limit of detection.4. An analysis software for the array SPR imaging sensor is developed. With the array SPRi sensing system, the basic performance of the sensor is tested. The dynamic range is about0.022RIU and the resolution is10-5RIU. The smallest change of light that can be detected is0.18greyscale bit and the repeatability is0.8053%. The measurement of BS A has been performed.Now the sensor can perform detection up to35spots, which has potiential for higer throuput and resolution.
Keywords/Search Tags:surface plasmon resonance, array sensor, SPRi sensor, high-thougput, polarization control method, HHT
PDF Full Text Request
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