| Study one Yin yellow card IETM model in ratsObjectives:Joint D-galactosamine (D-gal) intraperitoneal injection and rhubarb decoction lavage induced liver failure IETM Yin yellow card experimental animal model.Methods:68SD rats, were randomly divided into three groups, Ⅰ (normal control group), Ⅱ(yang yellow card group)and Ⅲ(Yin yellow card group). Normal group6, Ⅱ, Ⅲ groups each group only31. After experimental animal group, single cage, the free water, the same article3days, each animal weighing, measuring the anus temperature, from4days of the processing factors. Ⅱ group of rats, made mould to place it in hot and humid environment, Ⅲ group of rats, puts the cold dampness building environment. Group Ⅲ to400%rhubarb decoction according to the50ml/kg to fill the stomach,2times/day, Ⅱ group to1:15%sugar and pig oil mix20ml/kg to fill the stomach,2times/day; I set to saline to fill the stomach,2times/day. Hot and humid, cold dampness syndrome made mould for21days.21days will be D-gal intraperitoneal injection of1.8g/kg group Ⅱ and Ⅲ IETM liver failure model is established. By intraperitoneal injection of normal group amount of saline solution, After24h,48h were observed between groups of rats mortality, Liver function(ALT、AST、TBIL), Pathological changes of the liver tissue, Endotoxin level of portal vein, Rectum waste levels of intestinal flora. Results:1. General situation:group I rats without death. II, III group rats mortality respectively:20%,30%.2. Results of liver function:As a reference in group I, II and III group rats ALT, AST and TBIL were significantly increased, the difference was statistically significant (p<0.01). As a reference in group III, II group of ALT, AST and TBIL increased more obviously, the difference was statistically significant (p<0.05).3. Liver tissue pathology results:as a reference in group I, group III in the rat liver tissue damage is aggravating, obviously pathological integral is compared between each group, the difference was statistically significant (p<0.05). II, III group in the rat liver tissue damage to liver failure diagnosis of liver tissue damage level and liver tissue pathology the highest score in group III, comparing differences between two time points pathological integral group has no statistical significance (p>0.05).4. Portal vein endotoxin:As a reference in group I, II, III group rats with portal vein endotoxin obviously increases, the difference was statistically significant (p<0.01); Ⅱ, III group rats portal vein endotoxin comparative differences between groups was statistically significant (p<0.05).5.Excrement and urine levels of intestinal flora:As a reference in group I, enterococcus, Ⅱ, III group rats enterbacteriaceae were significantly increased, the difference was statistically significant (p<0.01); Bifidobacterium and lactobacillus were reduced, the difference was statistically significant (p<0.05);Ⅱ, III group comparison between groups of rats, enterococcus, e. coli and bifidobacteria difference was statistically significant (p<0.05), the difference of lactobacillus has no statistical significance (p>0.05).Conclussion:Combined cold dampness building environment, rhubarb decoction lavage and intraperitoneal injection of D-gal method to induce Yin yellow card IETM model is set up. Study two This toxin and blood endotoxin of IETM Yin yellow card liver failure rats, the influence of cytokinesObjectives:1.Research explored blood detoxification method of IETM Yin yellow card liver failure rat IL-6, C-met immune protein expression and expression.2. Study this toxin and blood Yin yellow card to liver failure after intervention IETM rat intestinal flora, the change of plasma endotoxin.Methods:86SD rats, Were randomly divided into Yin yellow card to liver failure IETM model group (group I), explored the detoxification of blood low dose group (group II), explored the detoxification of blood in the dose group (group III), explored the detoxifyication of blood high dose group (group IV), each group of20, Normal6(group V) in rats. II, III, IV group6days prior to the building of rats with different blood party explored detoxified dose lavage, I group of rats with normal saline lavage,2times/day, until death. Building21days in group I, II, III, IV to D-gal intraperitoneal injection of1.8g/kg Yin yellow card IETM model is set up; V group rats normal diet. Observed between groups of rats in24h,48h after building mortality, groups of rats and liver function (ALT, AST,TBIL) and liver tissue pathology, plasma endotoxin, intestinal flora, IL-6, C-the liver tissue tested met the immune protein expression and so on.Results: 1.Mortality:I group rats mortality rate of32%; Ⅱ, Ⅲ, Ⅳ group rats mortality declined gradually with the increase of this blood detoxification party dose, groups of mortality was23%,20%and15%, respectively.2. Liver function:compared with group Ⅰ, Ⅱ, Ⅲ, IV group rats ALT, AST, TBIL is reduced, the IV group and the comparison between the way, I was statistically significant difference (p<0.01).3. Liver tissue pathology results:compared with group Ⅰ, Ⅱ, Ⅲ, Ⅳ group, according to the results of liver tissue pathology damage reduce, only IV group and the group I liver histopathologic grading, the difference was statistically significant (p<0.05).4. Plasma endotoxin:compared with group Ⅰ, Ⅱ, Ⅲ, IV group, the level of endotoxin was reduced, only high dose group and the difference between model way was statistically significant (p<0.05).5. Fecal intestinal flora level:compared with group Ⅰ, Ⅱ, Ⅲ, Ⅳ group rat intestinal bacteria, e. coli were increased, the difference was statistically significant(p<0.01); BifidoBacterium and lactobacillus were reduced, the difference was statistically significant (p<0.05); Ⅱ, Ⅲ and IV respectiveely compared between group rats, enterococcus, e. coli and bifidobacteria difference was statistically significant (p<0.05), the difference of lactobacillus has no statistical significance (p>0.05). Group Ⅱ, Ⅲ, comparing differences between way has no statistical significance (p>0.05).6. IL-6level changes:the liver tissue compared with group Ⅰ, Ⅱ, Ⅲ, Ⅳ group rats IL-6levels increased significantly, the difference was statistically significant (p<0.05).7. C-met protein immunohistochemical:compared with group Ⅰ, Ⅱ, Ⅲ, IV group. C-met positive area were significantly increased, the difference was statistically significant (P<0.01); IV group compared with two Ⅱ, Ⅲ, IV group. C-met positive area is higher than Ⅱ, Ⅲ, the difference was statistically significant (P<0.05).Conclussion:1. Using this blood detoxification reduce liver failure IETM Yin yellow card mortality in rats, improve liver function, reduce liver tissue pathological situation.2. This blood detoxification side effects on liver failure mechanism of IETM Yin yellow card may be by (1) reduce the absorption of the plasma endotoxin;(2) inducing immune signal activation; To promote the increment of liver cell;(3) regulating intestinal flora; To implement. Study three Wenyang Jiedu Huayu method for patients with hepatitis B liver failure correlation Yin yellow card and Yin Yang yellow cardObjectives:Through selected60cases of patients with hepatitis B liver failure correlation the analysis of the combined therapy of Chinese and western, evaluate this toxin and blood in patients with hepatitis B liver failure correlation Yin yellow card and Yin Yang yellow card curative effect.Methods:Selected cases according to syndrome differentiation of TCM into the Yin yellow card and Yin Yang yellow card groups. And according to random number table is divided into the experimental group and control group, two groups of patients were in normal liver, protection of liver, stomach, improve the hepatic microcirculation, antiviral, symptomatic treatment, etc.on the basis of the experimental group to this blood detoxification granules, the control group on the basis of the simulation agent treatment with traditional Chinese medicine. After eight weeks, to evaluate curative effect of two groups of patients.Results:The two groups after treatment of liver TBIL, PTA, gut endotoxin comparison between groups, such as difference was statistically significant (P>0.05), inside two way comparison before and after the treatment, the difference was statistically significant (P<0.05).Conclussion:Combining this detoxification treated patients with hepatitis B liver failure correlation method, can improve symptoms, improve curative effect. |
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