Relationship Between CD36Single Nucleotide Polymorphisms And TCM Syndrome Type Of Diabetes

Objective： To explore the relationship between CD36single nucleotidepolymorphisms and TCM syndrome type of diabetes.Methods: Study1:405patients with diabetes which were divided into four groups:extreme heat with yin asthenia type, wet and heat puzzling the spleen type, Qi and Yindeficiency with blood stasis type and Yin-Yang deficiency type. The control group selectednon-diabetes physical examination person539cases. Two loci of CD36rs3211842,rs1761667individual genotypes were detected using polymerase chain reaction-ligasedetection reaction (PCR-LDR). Enzyme linked immunosorbent assay (ELISA) was usedfor determination of plasma thromboxane B2(TXB2).Study2: This study included107patients of Qi and Yin deficiency with blood stasistype diabetes accompany with atherosclerotic cerebral infarction.267patients withatherosclerotic cerebral infarction of the same TCM syndrome type is the control group.The methods is the same of study1.Study3: This study included107patients of diabetes accompany with atheroscleroticcerebral infarction.298patients with diabetes is the control group. The methods is thesame of study1.Results：Study1: There were no significant differences in the rs3211842andrs1761667genotype frequencies and allele frequencies between the four groups andcontrols（P＞0.05）. The platelet counts and plasma TXB2levels is significant differencesbetween of Qi and Yin deficiency with blood stasis type and controls（P＜0.05）.Study2: The distribution of rs3211842genotype frequencies and allele frequenciesin the Qi and Yin deficiency with blood stasis type of diabetes with significant differences compare to the atherosclerotic cerebral infarction control (X2=6.358, P=0.043).Compared to GG, genotype AG+AA can increase the risk of diabetes with atheroscleroticcerebral infarction after multi-factor logistic analysis and stepwise regression analysis（OR=2.460，95%CI=1.197~5.054，P=0.014）. The GAgenotype in combination withAAgenotype showed a significant association with HDL, CH, TXB2(P＜0.05).Study3: The distribution of rs3211842AG genotype frequencies and A allelefrequencies in diabetes with atherosclerotic cerebral infarction was significant differencescompare to the diabetes control (P=0.047，P=0.011). The AG genotype in combinationwith AA genotype showed a significant association with TXB2level(P＜0.05) and increasethe risk of diabetes with atherosclerotic cerebral infarction after stepwise regressionanalysis(P＜0.05).Conclusions：CD36rs3211842, rs1761667is not associated with diabetes and TCMsyndrome type of diabetes in our cohort. CD36rs3211842is the independent risk factors ofdiabetes with atherosclerotic cerebral infarction. CD36SNPs have correlation with plateletfunction.