The Association Study Of MATN3Polymorphisms And Osteoporosis Phenotypes In Postmenopausal Women Of Han Nationality In Beijing

Objective:MATN3encodes extracellular matrix proteins, and might modulate chondrocyte differentiation. The aim of this study is to explore if MATN3polymorphisms influence bone mineral density (BMD), fracture, vertebral fracture, bone turnover and25(OH)VitD in postmenopausal women.Methods:1488postmenopausal women of Han nationality were randomly selected in Beijing. Fracture and vertebral fracture phenotypes were accertained by questionnaire and vertebral X-ray reading. BMD of lumber spine (L2-4), femoral neck (FN) and total hip were measured by dual energy X-ray absorptiometry (DXA). An automated Roche electrochemiluminescence system was used to test serum bone turnover and25(OH)VitD. Four tagging single nucleotide polymorphisms (tagSNPs) in MATN3were determined by TaqMan Pre-Designed SNP Genotyping Assays in Real-Time PCR System. We used multiple statistic methods to test the associations between SNP genotypes, haplotypes and osteoporosis phenotypes. We also analyzed correlations among bone turnover,25(OH)VitD, age, BMD, fracture and vertebral fracture.Results:1. Polymorphisms of rs1109663and rs6734005were significantly associated with total hip BMD (p=0.000-0.032and0.005-0.026, respectively), and the significance persisted even after correction to-multiple testing. The minor allele of rs6734005had protective effects for total hip BMD. The association of rs10178256and total hip BMD was also suggestive (p=0.009-0.037).Consistent with these results, haplotype GAC was significantly associated with total hip BMD (p=0.005-0.023), and p value remained significant after correction to multiple testing. GAC also exerted protective influence against hip osteoporosis.2. MATN3polymorphisms were not associated with β-CTX, P1NP or25(OH)VitD.3. The serum concentration of β-CTX and P1NP declined with aging and then rose up in postmenopausal women, and they were negatively correlated with BMD of lumber spine, FN and total hip. Serum25(OH)VitD level of postmenopausal women in Beijing was remarkably low (13.10±5.37ng/ml), and decreased with aging. We did not find correlation between bone turnover,25(OH)VitD and fracture or vertebral fracture.Conclusion:This study suggested for the first time that MATN3polymorphisms might influence total hip BMD variation in postmenopausal women, and it was not related to bone turnover or25(OH)VitD.