| Blood, as the most important body fluid, contains lots of proteins secreted by organs or leaked from dead cells. To some extent, blood is a window of the body. During the fetal development, especially the midanaphase, each organ changes a lot in morphous and functions. Based on LC-MS/MS high throughput proteomics technologies, proteins as function performer are investagated. Analysing plasma or serum proteome of different period in fetal development, may have a better understanding biology of life.Considering the diversity of rat fetus in blood, pooled serum specimens and individual serum specimens from E15.5fetus and newborn rat were collected and analyzed using LC-MS/MS technology. Using three times LC-MS/MS analysis of same pooled specimens as instrumental variance control, The ratio of individual samples’ coefficient variation vs pooled samples’coefficient variation were used to reflect the diversity of individual samples in proteomics level.Using LC-MS/MS,669proteins were identified in the individual specimens with the confidence of99%. After two tailed T test, with95%confidence, from15.5d fetus to new born rat,35proteins increased and58decreased significantly. The functional analysis reveals that all9identified complement molecules increased, and6increased significantly.13enzyme regulators increased while4decreased significantly,8of11apolipoproteins decreased significantly. Hemoglobin species pattern were different at the two stages.Protein modifications can change the protein’s structure and functions. We found50different modifications in pooled specimens of the two stages. Some proteins are differentially modifid. This provides clues for the studying function of the modificaion in these serum proteins in the future. |
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