The Study On The Expression Patterns Of Genes Coding(D)C/AMPD And The Function Of Os05G0349200in Rice

Rice (Oryza sativa L.) is one of major food crops, which feeds about half of the world’s population. Systematic analysis of rice genes’function is important for molecular breeding in rice.We reported identification of rice deoxycytidine/cytidine deamina.se(CMPD) and adenosine/AMP deaminase (AMPD) families in our previous studies. Most genes from CMPD/AMPD families are differentially expressed during the rice-pathogen (Magnaporthe oryzae) interaction, and some of them are upregulated after blast inoculation. Functional studies of CMPD/AMPD families are even not reported in the model plant Arabidopsis. In this dissertation, we selected several genes from CMPD/AMPD families and detected their expression patterns under different biotic and abiotic stresses, and also generated RNAi transgenic rice plants to study one of AMPD’s function.We found that almost all of the selected genes were differentially expressed in the biotic and abiotic stressed plants compared with the control plants. Especially, the expression of the two genes, i.e., Os07g0245100(CMPD family) and Os05g0349200(AMPD family), was greatly upregulated under both the biotic and abiotic stresses, suggesting that these genes are involved in the response pathways related to biotic and abiotic stresses. In addition, the characterization of three independent RNAi tansgenic lines for the AMPD gene Os05g0349200showed that it may play an important role against blast and bacterial blight, as well as in rice development.All together, our study provides the first resport of a systematic analysis of the rice CMPD and AMPD families, the expression patterns of these genes in response to both biotic and abiotic stresses. Most important, the AMPD gene Os05g0349200has been proved to be against blast and bacterial blight in rice. In addition, the tansgenic rice plants generated in this study could be useful for further study of the AMPD gene.