Study On The Chemical Constituents Of Marine Sponges Xestospongia Testudinaria And Spheciospongia Sp. From The South China Sea

Marine sponge,which belongs to Porifera,is the most original multicellular marine organism.A great deal of research works on marine sponges revealed that the secondary metabolites of marine sponge possess remarkable biological activities,such as anti-bacterial,anti-tumor(cytotoxic),anti-fungus,anti-virus,anti-inflammation activities,and so on.In order to find natural products with the unique structures and promissing biological activities,two species sponges Xestospongia testudinaria and Spheciospongia sp.were collected from South China Sea near Sanya,Hainan province.Various of chromatography techniques were performed during the isolation and purification of the EtOAc extracts of Xestospongia testudinaria,as well as the PE and EtOAc extracts of sample of Spheciospongia sp.29 compounds were obtained from X.testudinaria and 22 compounds were isolated from Spheciospongia sp.Their structures were determined by extensive spectroscopic analysis in association with physical and chemical properties,as well as comparison of their spectral data with those reported in literatures.29 compounds isolated from the marine sponge Xestospongia testudinaria were identified as testudinlide A(1),testudinlide B(2),testudinlide C(3),testudinlide D(4), testudinlide E(5),testudinlide F(6),testudinlide G(7),testudinlide H(8),testudinlide I(9),testudinlide J(10),testudinlide K(11),testudinlide L(12),testudinlide M(13), testudinlide N(14),testudinlide O(15),testudinlide P(16),testudinlide Q(17), testudinlide R(18),testudinlide S(19),testudinlide Y(20),testudinlide U(21), testudinlide V(22),(7E,13E,15Z)- 14,16-dibromo-hexadeca-7,13,15-trien-5-yonic acid(23),(7E,13E,15Z)-14,16-dibromo-hexadeca-7,13,15-trien-5-yonic ethyl ester(24),indole-3-carboxaldehyde(25),indole-3-carboxylic acid(26), ethyl-indole-3- carboxylate(27),cyclo-(Ile-val)(28),cyclo-(Leu-val)(29) respectively.Among them,compounds 1-22 were new compounds.Compounds 1-23 were screend for their anti-tumor grouth and anti-HIV activities,however,they also showed unsignificant activity.20 compounds isolated from the marine sponge Spheciospongia sp.were identified as spheciospongone A(30),spheciospongone B(31),grasshopper ketone (32),3β-hydroxy-Sα,6α-epoxy-7-megastigmene-9-one(33),loliolide(34),cholesterol (35),cholest-4-en-3-one(36),cholest-3,6-dione(37),3β-hydroxy-cholest-5-en-7-one (38),6β-hydroxy-cholest-4-en-3-one(39),cholest-4-en-3,6-dione(40),cholest-5-en -3-one(41),cholest-3β,5α,6β-triol(42),6-methoxy-cholest-3β,5α-diol(43), 5-methoxy-cholest-3β,6β-diol(44),indole-3-acetamide(45),benzene acetamide(46), thymidine(47),uridine-N-methyl(48),2’-deoxyguanosine(49) respectively.Among them,compounds 30,31 were new compounds.On the base of anti-tumor bioactive screening,Compound 42 showed significant growth inhibitiory activity on REH cell line.This research work enriched the chemical constituents from marine sponge Xestospongia testudinaria and Spheciospongia sp.,and expanded the spectroscopy technique for structure elucidation of marine natural products,also enriched the chemical deversity for Chinese marine sponges.