| Section1 mirnas singgle nucleotide polymorpisms of liver cell cancer susceptibility and mir-196a2rs11614913 polymorpism relationship liver cancerObjective: The natural population based case-control study were chosen to reveal in the mi RNA coding region and targets of possible links between gene polymorphisms and the risk of liver cancer. Comprehensive analysis and environmental factors, genetic factors and mi RNA interaction effects on the risk of liver cancer. And according to the analysis of the genetic, cancer of the liver, to discuss different genotypes of micrornas in susceptible patients with hematoma of expression characteristics, provide reference for clinical for preventive diagnosis of liver cancer.Methods: 266 cases of hepatocellular carcinoma(HCC) patients with hepatocellular carcinoma(HCC) as experimental group were randomly selected from the first affiliated hospital of Inner mongolia medical university in September 2013- February 2013. 266 patients with no cancer control subjects after health check were randomly selected from experimental group of gender, age, hypertension, diabetes, smoking, drinking, infection, tumor size and tumor stage, and record all the control group after the three indicators were statisticed at the same time.5 ml peripheral venous blood samples were taken.Two groups of patients with venous Blood were collected and we use TIANamp Blood mi RNA to extract kit for mi R.Mi R- 146- a rs2910164, mi R- 196 a2 rs11614913, rs2292832 mi R- 149 and mi R- 499 rs3746444 genotype,by using restriction fragment length polymorphism polymerase chain reaction(PCR- RFLP). Results:(1) There was no statistically significant difference risk for between liver cancer with high blood pressure, diabetes, smoking, Drinking and virus infection were associated with a significant risk of liver cancer.(2) For the mi RNA in the coding region gene polymorphism and the risk of liver cancer relationship analysis results showed that mi R- 196(TT genotype and T allele gene) in the coding region gene polymorphism loci and the risk of developing liver cancer risk increased significantly, respectively increased by 2.29 times and 1.60 times(95% CI = 1.11 2.32), and mi R- 146, mi R- 149 and mi R- 499 polymorphism were not related with liver cancer.(3) HBV infection and mi R- 196 a2(CT + TT genotype) could be the reason for the increased risk of hepatocellular carcinoma(HCC).Gender, age and alcohol poisoning of mi R- 196 polymorphism and there could be no correlation between hepatocellular carcinoma(HCC) risk. Conclusion:(1)Mi R- 196 a2 rs11614913 TT genotype and T allele and were significantly correlated with HCC risk increases, as a symbol of the diagnosis of hepatocellular carcinoma(HCC).(2) HBV infection significantly influence mi R- 196 a2 rs11614913 polymorphism and had the relationship between hepatocellular carcinoma(HCC) risk.(3)mi R- 196 a2 rs11614913 TT genotype and allele T of hepatocellular carcinoma increas the risk.( 4) mi R- 196 a2 rs11614913 polymorphism may influence the development of hepatocellular carcinoma(HCC), especially in patients with HBV infection.Section 2 mir- 196 a2rs11614913 polymorphism research of relationship with the prognosis of liver cancerObjective: The study of first section could be found that f Mi R- 196- a polymorphism may be related to the patients with liver cancer, This section was to analysis Mi R- 196- a gene polymorphism and the correlation between the prognosis of primary liver cancer related indexes, we aim to guiding the treatment of hepatocellular carcinoma(HCC) chemotherapy drugs and evaluate the prognosis of patients and the survival time possible reference.Methods: 123 cases of hepatocellular carcinoma(HCC) patients with hepatocellular carcinoma(HCC) as experimental group were randomly selected from the first affiliated hospital of Inner mongolia medical university in September 2012- February 2013. Both groups are using polymerase chain reaction- restriction fragment length polymorphism method to detect the Mi R- 196 a2rs11614913 gene polymorphism. Gene counting method were inspected by two teams to allele frequency and genotype frequency. In the past used chemotherapy drugs. Drug treatment: hepatocellular carcinoma postoperative intravenous FOLFOX combination therapy, The curative effect of chemotherapy drug treatment, all patients were willing to chemotherapy after surgery, after preoperative baseline data were obtained from the clinical cases, patients discharged from hospital after every four weeks or outpatient follow-up telephone follow-up, until the death or the end of the study. Compared the baseline data, observation of Mi R- 196 type a2rs11614913 gene polymorphism.Results : 123 cases of breast cancer patients, 90(73.2%) of the tumor were less than5 cm in diameter, 33(26.8%), tumors were larger than 5 cm in diameter, 47(38.2%) showed the TNM staging of tumor the II, 76(61.8%) were III- IV, in continuous follow-up, Mi R- 196 a2rs11614913 genotypes and the correlation of chemotherapeutic drugs were sensitivity. By chi-square test, we found that Mi R- 196 a2rs11614913 genotypes in chemotherapy drug were sensitive group and insensitive group were statistically significant difference between(chi-square = 11.91, P = 0.001). Mi R- 196 a2rs11614913 genotypes and the correlation of liver cancer mortality Cox proportional hazards regression model, a genetic defect type significantly reduced the risk of liver cancer died and HR(95% CI) was 0.49(0.25 0.96).Conclusion:(1)Mi R- 196 a2rs11614913 polymorphism associated with patients’ drug sensitivity to chemotherapy and survival time. While compared with a2rs11614913 functional Mi R- 196, Mi R- 196 a2rs11614913 defect type genotype of breast cancer patients were more sensitive to chemotherapy drugs.(2)Mi R- 196 a2rs11614913 genotypes in patients with genotype were short lifetime.(3)Mi R- 196 a2rs11614913 polymorphism in patients with positive could provide reference for individualized chemotherapy regimens for patients. |
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