The Expression And Biological Function Of Long Noncoding RNA Linc00467 And ZFAS1 In Human Sporadic Colorectal Cancer

BackgroundColorectal cancer is one of the most common tumors. The incidence and mortality of colorectal cancer in our country rank top fifth in all tumors. New oncogenes and tumor suppressor gene are involved in the occurrence and development of colorectal cancer. Encyclopedia of DNA elements in the human genome project revealed that only 2% genes are protein coding genes, the rest of genes are non-protein coding genes. According to the number of nucleotides, non-protein coding RNA are divided into long noncoding RNA and small noncoding RNA. It has been found that some lncRNA has some effect on the occurrence and development of tumor.ObjectiveOur study was performed to explore the expression and function of lncRNA Linc00467 and ZFAS1 in colorectal cancer.MethodsFirst of all, we examined the expression patterns of 25 lncRNA in 11 pairs of colon rectal cancer tissues and adjacent normal tissues by real-time PCR and determine Linc00467 and ZFAS1 as the target genes to be studied according to the relative expression differences.The relative expression level of Linc00467 in colorectal cancer tissues, adjacent normal tissues, normal colon cells and colon cancer cells were detected by real-time PCR. 67 patients of colorectal cancer were selected and their clinicopathological characteristics were collected. The relationship between the expression level of Linc00467 and the clinicopathological features of the patients was analyzed. The effect of Linc00467 on migration and proliferation of colon cancer cells was detected by Transwell migration assay, MTS assay and clone formation assay.The relative expression level of ZFAS1 in colorectal cancer tissues, adjacent normal tissues, normal colon cells and colon cancer cells were detected by real-time PCR.67 patients of colorectal cancer were selected and their clinicopathological characteristics were collected. The relationship between the expression level of ZFAS1 and the clinicopathological features of the patients was analyzed. The effect of ZFAS1 on migration and proliferation of colon cancer cells was detected by Transwell migration assay, MTS assay and clone formation assay. HCT-116, LoVo and SW480 cells were transfected with control siRNAs and Linc00467 siRNA to induce ZFAS1 knock down. Then detect the relative expression level of the potential target gene ZNFX1 of ZFAS1. Transwell migration assay, MTS assay and clone formation assay were used to examined the effect of ZNFX1 on migration and proliferation of colon cancer cellsResultsThe expression level of Linc00467 and ZFAS1 in human colorectal cancer tissues and colon cancer cells were significantly high compared with adjacent match-normal tissues and normal colonic epithelial cell line. There is no significant difference between the expression level of Linc00467 and ZFAS1 with clinicopathological characteristics of colorectal cancer. Linc00467 and ZFAS1 promoted CRC cell proliferation and migration. Knockdown of ZFAS1 caused an increase in the expression of target gene ZNFX1. The expression of ZNFX1 is down regulated in colon cancer cell line. Overexpression of ZNFX1 promoted CRC cell proliferation and migration.ConclusionsLinc00467 and ZFAS1 are highly expressed in colorectal cancer, which can promote the migration and proliferation of colon cancer cells. The function of ZFAS1 may be related to the potential target gene ZNFX1. Linc00467 and ZFAS1 may serve as a new biomarker in human colorectal cancer.