The Mechanisms Of Long Non-coding RNA 00152 In Gastric Progression

Gastric cancer (GC) was the fourth most common malignant tumor globally and it was a serious threat to human health, especially in developing countries. There was nearly one million new cases of gastric cancer in the world each year, about two-thirds of them from the developing countries and the occurrence in China accounted for 42%. Early diagnosis and treatment of gastric cancer was crucial for prognosis, and which was the main breakthrough directions in gastric cancer research. Abnormal regulation of multiple genes and multi-step progress involved in the development of gastric cancer, and finally result in the biological evolution of cells.The study found the occurrence and development of gastric cancer is closely linked with the gene. Comparing with the normal ratio in vivo,the specific gene associated gastric cancer had changed. The possible mutation genes is the key cause to stomach cancer. When abnormal behavior of the cell stemed from genetic mutation, it can lead to irreversible changes in cell structure and function, finally may result in carcinogenesis. Distant metastasis and recurrence was main causes of death in gastric cancer patients, and which also was the key factor affecting the clinical efficacy and prognosis. Depth study of the pathogenesis and the molecular mechanisms of metastasis of gastric cancer can provide a theoretical foundation for the exploration of new diagnostics and targeted therapy sites, also can provide the basis for early diagnosis and prognosis of gastric cancer.In the past, mechanisms studies in the development of gastric cancer mostly focused on traditional protein-coding genes, related to the regulation of genetic、 epigenetic and related signaling pathways. With the development of high-throughput sequencing technology, the researchers have revealed that the non-coded RNA (Non-coding RNA, ncRNA) accounted for more than 95% the proportion in the human genome, complex interplay between protein-coding RNA and functional non-coding RNA plays an important role in the occurrence and development of gastric cancer. Long non-coding RNA (long non-coding RNA, lncRNA), as the major component of ncRNA, attract more and more attention. Now it has been revealed that lncRNA participated in epigenetic, transcriptional and post-transcriptional regulation level in a variety of ways and has the potential for early disease diagnosis markers. In recent years, molecular mechanism of non-coding RNA especially the long non-coding RNA (Long non-coding RNA, lncRNA) in the tumorigenesis study recieved more and more attention. So far, there have been numerous reports about lncRNA highly correlated with proliferation, invasion and metastasis and recurrence of tumor. Similarly, in the gastric cancer study, a remarkable different expression profile of incRNAs was observed and some lncRNA, such as H19、HULC, which can regulate the proliferation and invasion and the development of gastric cancer by targeting downstream gene on different mechanisms. But the studies on lncRNA in gastric cancer still remain in infancy, new specific regulatory mechanisms on lncRNA can provide a new theoretical basis for the pathogenesis of gastric cancer. Long non-coding RNA transcript refers to a class of RNA, length greater than 200 nucleotides of, in the nucleus or cytoplasm, involved or not involved in the protein encoding, mainly participated in gene expression regulation in the epigenetic, transcriptional and post-transcriptional form. LncRNA mainly involved in genomic imprinting, chromosome silencing and chromatin modification, mRNA transcription activation, post-transcriptional interference, and transcriptional regulation, nuclear transport, a variety of important cis-or trans-regulation of proto-oncogene activation, it can activate or inhibit the expression of protein-coding genes.It was hopeful marker for early stage tumors。In our study, we used bioinformatic analysis, microarray combining classical molecular biology techniques to explore the biological behavior of the long non-coding RNA Linc00152 in gastric cancer. our chip results agreed with other researchers:the long non-coding Linc00152 had the maximum difference. Linc00152 expression in the72 cases of gastric carcinoma patients rendered consistent with the microarray expression using qRT-PCR. Comparing with gastric cancer patient clinical information, Linc00152 expression level in patients positively correlated with tumor size, not with the TNM stage and lymph node metastasis, cytoplasmic-nuclear isolation of RNA extraction method showed Linc00152 mainly in the cytoplasm and only a little in the nucleus. To clarify the non-coding sequence segment features with Long non-coding RNA, bioinformatics prediction methods was used to confirm the non-coding biological properties of Linc00152. To explore the knockout effect of Linc00152 shRNA cell function, we selected high expression cell lines MGC803 and HGC-27. CCK8 and EDU Proliferation assay was applied to explore relationship between Linc00152 and tumor growth.RNA Pull down experiments and mass spectrometry showed that the epidermal growth factor receptor was the protein Linc00152 captured. RNA immunoprecipitation (RNA Immunoprecipitation, RIP) further verified Linc00152 direct binding EGFR protein,thereby affecting their function and related effects. Final results show that Linc00152 may promoted cell growth and invasion of gastric cancer by EGFR related signal pathway and played an important role in the development of gastric cancer.In summary, our non-coding RNA functional study involved in the occurrence and development, especially growth and metastasis of gastric cancer. Gradually understand of complicated signal path process in a new perspectives may provide a solid theoretical foundation for early diagnosis and targeted treatment of gastric cancer in the future.