| IntroductionDental tissue-derived stem cells are stem stems separated from the dental tissue which have the potential of self proliferation and multi-directional differentiation potential. Dental pulp stem cells, periodontal ligament stem cells and Stem cells from the apical papilla are very important Dental tissue-derived stem cells which can be used in tissue engineering research. Many factors have important roles in regulating proliferation and differentiation of Dental tissue-derived stem cells. After researchers lucubrate on the regulatory effect of micro RNA on Dental tissue-derived stem cells, scholars discovered that many noncoding RNAs are key factors in regulating proliferation and differentiation of Dental tissue-derived stem cells.There are 93% DNAs translate into RNAs in the human genome, however thera are only 2% RNAs are transcript into protein. Most of the DNAs translate into noncoding RNAs. Long noncoding RNA used to be recognized as useless transcriptional noise. In recent years, scientist discovered that they are very important in the regulation of epigenetic, transcriptional and post-transcriptional gene expression. anti-differetiation noncoding RNA(ANCR) plays very important role in the regulation of cell differentiation. However, there are no reports on the regulatory function of ANCR on the differentiation of Dental tissue-derived stem cells. Our experiment uses RNA interfere to down-regulate the expression of ANCR in Dental tissue-derived stem cells in order to analyse the regulatory function of ANCR on the proliferation and differentiation of Dental tissue-derived stem cells. Main results 1. ANCR plays very important role in the regulation of osteogenesic, adipogenic and neurogenic differentiation in DPSCWe use RNAi to down regulate the gene expression of ANCR in DPSC. The down-regulation of ANCR has little effect on the proliferation of DPSC. However, the down-regulation of ANCR can promote the osteogenesic, adipogenic and neurogenic differentiation of DPSC and up-regulated related genes. The down-regulation of ANCR can remarkably enhance the formation of axon after the neurogenic induction of DPSC. 2. ANCR plays very important role in the regulation of proliferation and osteogenesic, adipogenic and neurogenic differentiation in PDLSCWe use RNAi to down regulate the gene expression of ANCR in PDLSC. The down-regulation of ANCR can promote the proliferation, osteogenesic, adipogenic and neurogenic differentiation of PDLSC and up-regulated related genes. The down-regulation of ANCR can activate canonical Wnt signal pathway by down-regulating the gene expression of GSK3-βwhich lead to the promotion of osteogenesic differentiation of PDLSC. Unlike DPSC, PDLSC looks more like neuron body after the downregulation of ANCR after the neurogenic induction. 3. ANCR plays very important role in the regulation of osteogenesic, adipogenic differentiation in SCAPWe use RNAi to down regulate the gene expression of ANCR in SCAP. The down-regulation of ANCR has little effect on the proliferation of SCAP. However, the down-regulation of ANCR can promote the osteogenesic and adipogenic differentiation of SCAP and up-regulated related genes. The down-regulation of ANCR can remarkably enhance the formation of axon after the neurogenic induction of DPSC. The down-regulation of ANCR has little effect on the neurogenic differentiation of SCAP. ConclussionsIn summary, ANCR plays very important role in the regulation of proliferation and differentiation of Dental tissue-derived stem cells(DPSC, PDLSC and SCAP). However, the regulatory effect of ANCR on these stem cells is different. The down-regulation of ANCR can promote the osteogenesic, adipogenic and neurogenic differentiation of DPSC, the proliferation, osteogenesic, adipogenic and neurogenic differentiation of PDLSC and the osteogenesic and adipogenic differentiation of SCAP. The down-regulation of ANCR can activate canonical Wnt signal pathway which lead to the promotion of osteogenesic differentiation of PDLSC. |
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