Experimental Study On The Effects Of Magnetic Nanoparticles In The Diagnosis And Treatment Of Ovarian Carcinoma

Ovarian cancer,one of the most common gynecologic malignancies, is the third leading cause of death and has the highest mortality rate among the gynecologic malignancies.Its five-year survival rate rambled between30%-40%.The main reasons are the difficult diagnosis to early tumor and primary or acguired drug resistance after chemotherapy.The diagnosis to early ovarian tumor is so difficult that70%patients were in advanced stage upon diagnosis. The main treatment for these patients of late stages is still combined therapy which based on surgery and with chemotherapy, radiotherapy and immuno biological therapy. However, surgical treatment is difficult to obtain good effect in ovarian cancer,cytoreductive surgery couldn’t be achieved in large number of most advanced cancer even lose the chance of surgery, because of widespread metastasis.so chemotherapy in cancer treatment is increasingly important.Although the chemotherapy schemes has become more reasonable, the survival rate of patients with ovarian carcinoma is of a high level.The reasons are as follows:Traditional chemotherapy drugs and administration approaches also exist inevitable shortcomings, such that the medicine lacks the specific; drug metabolism in the body need several barrier; only a few chemotherapy drugs can reach the tumour site; traditional administration way make the drug uniformly distributed over the whole body, and produce toxic side effects to the general cells; primary and secondary resistance can influence the effect of chemotherapy.So how to enhance early diagnostic rate and improve the sensitivity of chemotherapy agents is going to as one of the important directions of tumor study.In recent years, nanomaterials are used in more and more specific areas because of their unique structure, composition, and properties.As Nano-material has strong drug loading capacity, and the function of linking with a variety of anti-cancer drug、 radioactive element、bioactive protein and low-molecular multi-peptide,by using different types of carrier preparation, further combine with antibodies with affinity in pathological tissues or cells, and delivering chemical drg、nucleic acid carriers to target spots to produce therapy functions, so as to improve the function、reduce toxicity、enhance early diagnostic rate, and it is expected to become a novel drug delivery system.This paper is focused on the application of nanomaterials as drug delivery on the diagnosis and treatment for ovarian cancer, which are demonstrated as follows:1. Synthesizing a new nuclear magnetic resonance contrast medium and connecting the modified folic acid compounds and gadolinium compounds with it,so as to enhancing its characteristics of targeting and high organization resolution.2. The contrast medium was characteristiced and detected, including cytotoxicity, water solubility, stability, targeting and high organization resolution etc.3. Synthesizing and functionalizing single-walled carbon nanotubes, and make it carried in propidium iodide and cisplatin, by testing the cytotoxicity increasing and apoptosis inducing, after cisplatin-carbon nanotube delivery system acts on cervical carcinoma cell lines Helacells, probe into the possibility that single-wall carbon nanotube can enhance chemotherapy as chemotherapeutic drugs carriers.4. The Cisplatin-carbon nanotube system which was mentioned above was acted on the ovary cancer cell which is drug tolerance to Cisplatin, through detecting cytotoxicity, concentration of intra-cellular carrier and the amount of cisplatin, the possibility and themechanism of the nanotubes as drug carrier to cure chemotherapy resistance drug fast tumour were investigated.The conclution is that:The experiment synthesized a new nuclear magnetic resonance contrast medium with target of ovarian cancer cells, and can increase the nuclear magnetic resonance imaging(MRI) organization resolution; This experiment in the synthesis of large diameter carbon nanotubes as the carrier of cisplatin, can increase cisplatin into HeLa cells and drug resistance COC1ovarian cancer cell volume, thus increasing the damage to cells cisplatin toxicity. The method for the next step synthesizing set diagnosis and treatment for the integration of multi-function carrier provides theory and the technology basis.