The Comparative Analysis Of Relevant Immune Factors In Breast Tissue And Peripheral Blood From Early Breast Cancer Patients

Objective:Immune microenvironment plays an important role in the development and progression of breast cancer. We test the expression of IL-2, IL-6, TGF β-1, HIF-1 a and PD-L1 in the tumor tissue and the peripheral blood of the patients with breast cancer.Through analyzing the difference and correlation of these immune factors expression between the tumor tissue and the peripheral blood.we try to found somg immune factors in tumor immune microenvironment which played important role in the development and metastasis of breast ancer. In addition, detection of peripheral blood immune factors can be used to monitor the curative effect and changes of immune function of the breast cancer patients.Methods:The tissue and blood samples were derived from breast cancer patients who had accepted the operation treatment between 2013-6～2014-06 in 301 hospital, and all of their pathologically diagnoses are confirmed. The tissue samples were tested by immunohistochemistry (IHC) and the blood samples were tested by ELISA. Every sample was tested with the following targets:IL-2, IL-6, TGF-β1, HIF-1α and PD-L1. Then we use the statistical analysis technique to analysis these results and clarify the correlation between these immune factors.Results:1. Analyzing immune factors of the peripheral blood, the expression of IL-2 protein content for breast cancer patients was lower than healthy group (P<0.01), but TGF β-1 was higher (P<0.01);2.The subgroups analyzing of the peripheral blood results of immune factors for patients with clinical pathological feature showed that:the expression of IL-6 was higher in III stage patients than in Ⅱ and I stage patients (P values were 0.027, 0.042 and 0.003, respectively). The expression of PD-L1 in the peripheral blood were higher in triple negative breast cancer patients. (P values were 0.027).3.The subgroups analyzing of the immunohistochemical results of immune factors for patients with different clinical pathological feature showed that:the IL-6 positive expression rate is higher in in triple negative breast cancer(P=0.040). TGF-β1 in in tumor tissue is correlated with age; The expression of IL-6 in tumor tissue was higher in menopause patients (P=0.01) and obese patients (P=0.02)4.The correlation analyzing of the immune factors between immunohistochemical results and peripheral blood results showed that:the level of TGF-β1 in peripheral blood are positive correlated with their expression in tumor tissue (Correlation coefficient were 0.415, respectively; P values were 0.01); other immune factors don’t show statistical correlation (P>0.05). The level of IL-6 and PD-L1 in peripheral blood are positive correlated with expression of TGF-β1 in tumor tissue (Correlation coefficient were 0.312and 0.284, P value were 0.018 and 0.032,); The expression of IL-2 in tumor tissue is positive correlated with expression of TGF-β1 in rumor tissue (Correlation coefficient was 0.288, P value was 0.032);5. The expressions of TGF-β1、CTLA-4 and IL-6 in the peripheral blood of patients decreased after operation.Conclusion:1.The immune function in Breast cancer patients was suppressed. Immunosuppressive factor TGF-β1 increased and IL-2 decreased.2.Operation improve immune function.The level of IL-6, TGF-β1 and CTLA-4 in peripheral blood decreased in the patients after the operation.3. The expressions of IL-6 in tumor tissues expression was higher in triple negative breast cancer.4. The expression of IL-6 in peripheral blood was associated with the clinical staging of tumor; The expressions of PD-L1 in peripheral blood was higher in triple negative breast cancer.5. The expression levels of TGFβ-1 in peripheral blood can reflect the expression of TGFβ-1 in tumor immune microenvironment.