Simulated Prepartion Of Soybean Protein For Biomedical Materials

By the combined of Aspen(?) series and Fluent(?0 series simulation platform for calculation in reverse micelles method producing soybean protein industrialization process, the simulation method of the chemical industry is used in food industry. The simulation software Aspen Plus(?), computational fluid dynamics simulation software Fluent(?)and Mixsim were used for the main unit operations involved in the actual production to carry out the design and calculation, including the mixing tank, pump, centrifuge, distillation tower, the reboiler and condenser.On the based of steady state simulation,dynamic simulation was used for simulation in the process of key equipment of rectification tower in the actual industrial production about start, operation and stop process which may exist the feed flow rate and feed composition disturbance and other special circumstances.The analysis and research of the product purity robustness,the anti-disturbance ability and response effect on abnormal situations were discussed.At the same time, by reverse micelles extraction,soybean oil can be extracted and the extraction solvent can be recycling used.Through the combination of various computing simulation platform,the method of reverse micelle extraction of soybean protein was used in actual industrial factories.In this paper, by reverse micelles produced soybean protein were used as raw material,through layer by layer self-assembly technology, combined with poly-lysine, prepared on soybean protein microtub.It can be to the localization of HeLa cells for biodegradation and targeting.By scanning electron microscopy, transmission electron microscopy, fluorescence microscopy and energy dispersive X-ray spectra technology to confirm the formation of microtube,biodegradable, directional magnetron and photothermal conversion properties.Soybean protein biodegradable microtube is a new type of photothermal therapy agent, and it explores a new method and feasibility of photothermal method for the treatment of cancer.The main results are as follows:The steady-state industrial process analysis in reverse micelles extraction of soybean protein was based on simulation in Aspen Plus and Fluent platform.Simulation calculation of key parameters for Aspen Plus software, modeling software Gambit, agitator tank special design software Mixsim, computational fluid dynamics software Fluent to simulate the main equipment calculation by reverse micelles extraction of soybean protein in the industrial production process using the steady state, draw out the industrial simulation of the production flow chart, and the main design parameters:Regional fluid velocity vector, stirred tank simulated by single and double blade turbine impeller rotational speed corresponding to different can be obtained:paddle type stirring paddle should use turbine agitator; stirred tank through on different impeller diameters than (inner diameter ratio of diameter of stirring paddle with the mixing tank) simulation, values can be obtained:when the stirring tank of impeller diameter ratio was 1:3 mixing effect is better; the simulation calculation of stirring paddle placed in different turbulent energy curves of depth can be seen, y=0.5H (depth of mixing tank placed actual high degree half) when the stirring effect can be achieved better. Stirring under the above conditions, the required stirring time is 43min, the motor power is 4936W, the pressure nephogram display corresponding stirring paddle stirring paddle, the leaf margin bear larger pressure, up to 3.92×104Pa (relative pressure), stirred tank parameters for the corresponding:stirring paddle impeller diameter is 0.65m width 0.11m of the impeller, impeller from the mixing tank bottom height 1.75m, stirring speed,160r/min, blade number 4, number 4 baffle, baffle width 0.3m, agitation tank, the effective volume 9m3, stirred tank diameter 1.8m, stirring the pot body actual height 3.5m.Using Aspen Plus software to simulate calculation of pump,calculated by using physical methods for PENG-ROB, simulation calculation:pump-1 -2 -3 -4,head 40.8m,40.8m,32.4m and 28.7m,NPSH required to meet the 0.91m,0.91m,0.51m and 0.43m, shaft power pump need to meet 6.2kW,6.2kW,5.3kW and 4.7kW.Pump body by using Gambit software for an meshing, simulation, the 4 pump bodies after gridding model were introduced into Fluent software for pump speed and pressure field was obtained.Pump-1 and pump-2 volute turning fluid were high speed, wide pressure pump-3 was relatively uniform, edge pressure pump-4 the volute was big.Centrifuge simulation models using horizontal spiral unloading settling centrifuge. For -1 and-2 parameters of centrifuge centrifuge by using HyCyc module of Aspen Plus software simulation, get the pressure centrifuge centrifuge -1 and -2 drop were 15kPa and 22kPa, the body length were 1.8m and 2.6m, the body diameter are respectively 0.65m and 0.95m, the body inlet diameter were 0.24m and 0.36m, the body overflow the diameter of the mouth are respectively 0.21m and 0.25m, the body underflow mouth diameter are respectively 0.08m and 0.17m.The principle of distillation tower was drew sketch, through the sensitivity of Aspen Plus software calculation and analysis module, can be drew:when the vacuum distillation reflux ratio 0.352, top distillate flow rate is 3800L/h, the quality of oil and solvent purity can respectively reach 98.9% and 99.7%, with the increase of rectifying tower plate number. The purity of the oils and solvents increase respectively, and identified the distillation tower with 7 theoretical plates.The reboiler and condenser using Exchanger Design Rating (EDR) software and Aspen Plus software to carry on simulation calculation of again reboiler and condenser,we obtained the assembly plan reboiler and condenser and piping figure, and accounting of the reboiler and condenser of quality and cost.Shell side inlet and outlet diameter and tube side inlet and outlet diameter, moreover the temperature distribution of shell and tube were simulated.Dynamic industrial process analysis in reverse micelles extraction of soybean protein was based on Dynamics platform.The robustness of the simulation software Aspen Dynamics was investigated by reverse micelles key equipment-flow process of soybean protein production in the distillation column using dynamic simulation, and the dynamic stability of the rectifying tower and anti disturbance performance calculation, we obtain the following parameters:Through the relationship between the calculation of distillation tower, tower top and bottom product quality and purity with the change of production time in distillation column startup process can be drew:when the distillation tower after starting 45min, tower top and bottom products quality purity reached the target of purity. When the distillation column feed flow rate than the original feed flow rate increases 20%, the top and bottom of the column product quality and purity variations were larger, respectively in the disturbance after the addition of 50min and 35min after the re reach stable; when the distillation column feed flow rate than the original feed flow is reduced 20%, tower top and bottom products change is not the quality of purity, were renewed in the 40min after the addition of the disturbance and after 35min reaches stable, the fluctuation amplitude is very small, the waveform is not obvious; when the distillation tower feeding occurred +5% disturbance component, the top and bottom of the column product quality and purity changes greatly, graphic wave obvious; when the feed to the column occurs-5% disturbance component, the top and bottom of the column product quality and purity change is small, the graphics fluctuation was not obvious. It can be seen that the feed composition disturbances is more obvious than the feed flow rate disturbances caused a big influence to the startup process, increase the distillation tower overhead and bottom products quality purity increases gradually with time.In the process of distillation tower to stop when the stop after 10min, distillation tower top and bottom product quality and purity start did not meet design requirements, namely the distillation tower stop operation within 10min of the top and bottom of the column products are qualified products. Changes in the process of distillation tower to stop operation of tower top and bottom product quality and purity decreased gradually with time.Preparation process of soybean protein microtube can be optimized.Through electrostatic interaction, poly-lysine and soybean protein can be prepared on microtube. Using the rate of microtube preparation as the index.Plackett-Burman, steepest ascent and response surface design optimize the microtube preparation process, parameters of biodegradable microtube conditions is protein concentration:8.52%, poly-lysine concentration: 6.32%, poly-lysine immersion time:20.2min, preparing temperature 31.4℃.Under this condition, the theory preparing rate of microtube can reach 77.28%.The actual rate was 76.82%.Therefore, the Plackett-Burman, the steepest ascent and response surface design for soybean protein biodegradable microtube preparation process optimized is accurate and reliable.Soybean protein Microtube can be controlled and thermal effect.Au nanoparticles assembly outer surface on the biodegradation of microtube in soybean protein, irradiated with NIR, Au nanoparticles can convert light into heat energy.Fe3O4 nanoparticle assembly in microtubules within the surface, through the external magnetic field to locate and manipulate the microtube, and the microtube can be located near the HeLa cells. Photothermal conversion experiment can prove that, in the presence of NIR light, microtube by photothermal effect can improve the microtube suspension temperature more than 42 ℃, and the temperature can kill cancer cells, at the same time by fluorescence image can be seen, the photothermal effect of microtube can effectively kill HeLa cells, induced apoptosis of HeLa cells. Blank test showed that HeLa cells were killed by microtube photothermal effect, and increased with the concentration of microtube, near the temperature continuously upgrade.By dynamic light scattering experiments, we can see that the size distribution of microtube, the average particle size in the 15-20μm were the most common, accounting for 78%. In addition, the composition of the microtubue is soybean protein, through enzyme treatment microtube can be degraded, the microtube is biodegradable. Because of targeting property, biodegradability, easily controlling, soybean protein biodegradable microtube has the great development potential and application prospects in the field of photodynamic therapy.