Effect Of CD4~+CD25~+Regulatory T Cells And T Helper Type 17 Cells In Septic Rats

Objective To investigate the roles of CD4+CD25+regulatory T cells (Treg cells) and Th17 cells in inflammatory response in septic rats, to further explore the pathogenesis of disturbed adaptive immune response in rats with sepsis. Methods A total of 110 healthy male Sprague-Dawley rats(250-300g) were randomly divided into 3 groups: normal control group, sham group and CLP model group. The rat models of sepsis were established by CLP. Flow cytometric analysis(FCM) was performed to detect the percentage of CD4+T cell, Th17 cells and Treg cells subpopulation. Expressions of proinfiammatory cytokines[IL-6, IL-10, TNF-α, TGF-β, IL-17] were measured by using enzyme-linked immunosorbent serologic assay. Acording to IL-10/TNF-α and HLA-DR expression of CD14+ monocyte to evaluate immune response in rats with sepsis (>30% or<30%). Results Detected by enzyme-linked immunosorbent serologic assay and flow cytometric analysis, the ratio of IL-10/TNF-a and the proportion of HLA-DR was higher than that in control group. Compared with sham group, While the spesis becoming worse, the rats were immunological inhibition. The proportion of Treg cells was found to be significantly lower than that in control group (P<0.01), while with the development of the sepsis, the proportions of Treg cells in sepsis group were significantly higher. The proportion of Thl7 ceils were significantly decreased in control group (P<0.01), while with the development of the sepsis, the proportions of Thl7 ceils in sepsis group were significantly higher. Expression levels of IL-6, IL-10, TNF-a, TGF-0, IL-17 were significantly up-regulated in sepsis group. Conclusion While the spesis becoming worse, the rats were immunological inhibition. Aberrant activation of Th17 cells and Treg cells might be involved in pathogenesis in sepsis. The changes of cytokine environment in sepsis may be one of the factors causing the imbalance of Treg cells/Th17 cells.Objective To investigate the effect of thymosin al on Thl7 cells in rats with sepsis, to further explore the Thymosin al and Thl 7 cells in pathogenesis of disturbed adaptive immune response in rats with sepsis. Methods A total of 110 healthy male Sprague-Dawley rats(250-300g) were randomly divided into 5 groups: normal control group,TPN+Talgroup, TPN group, TPN+Tal+CLP group? TPN+CLP group.The rat models of sepsis were established by CLP. Flow cytometric analysis(FCM) was performed to detect the percentage of Thl7 cells. Expressions of proinflammatory cytokinesIL-17 were measured by using enzyme-linked immunosorbent serologic assay. We observed the effect of thymosin al on mortality, Thl 7 cells,IL-17, albumin and C-reactive pritein(CRP) in reforming cecal ligation and puncture(CLP) induced septic rats. The MAP droped to 70% of the baseline was defined as septic shock. Results Thymosin al reduced cvumnulative 72 h mortality.Concentration of Thl 7 cells and IL-17 in reforming CLP induced septic rats increaseed significantly compared to control group. Thymosin al significantly decreaseed Thl7 cells and IL-17 concentotion in reforming CLP induced septic rats. Thymosin al reduced degressive degree of albumin, Concentration of CRP increased in both sepsis groups, but was less prominently in thymosin al treated group. Conclusion Thymosin al can improve immunological function, inflammation condition and protein metabolism throuth Thl 7 cells and IL-17.