| Objective: Observe the effects of Bi Xie aqueous extract to uric acid nephropathy raton the expression of inflammatory cytokines, such as tumor necrosis factor-α, monocytechemotactic protein-1and intercellular adhesion molecule-1. And blend Bi Xie withSmilax glabra, Coix seed, Morinda officinalis and the bark of ash made into compound BiXie soup applied on uric acid nephropathy rats, and observe the expression of urineneutrophils gelatinases related lipid carrier protein and Kidney injury molecule-1. Applycompound Bi Xie soup to uric acid nephropathy patients and observe the clinical curativeeffect. Analyze the protection mechanism in kidney disease of Bi Xie aqueous extract andits main component dioscin in uric acid nephropathy animal models.Methods: Apply Oteracil potassium to make animal model of uric acid nephropathy.Randomly divide into control group, allopurinol group, low dose, middle dose and highdose drug intervention group. During drug intervention regular test the uric acid, ureanitrogen, creatinine and monocyte chemotactic protein1level in serum. Before24h ofblood collection, collect24h urine to detect NGAL and KIM-1content in urine urine. atthe end of the experiment, detect the expression of tumor necrosis factor alpha, monocytechemotactic protein-1and intercellular adhesion molecule-1in kidney tissue of each grouprat by real-time PCR method. Apply compound Bi Xie soup in clinical practice46casesuric acid nephropathy patients were randomly divided into the compound Bi Xie decoctiongroup (n=24) and allopurinol group (n=22), before treatment test and after8weekstreatment test serum uric acid, urea nitrogen, creatinine levels. And collect24h urine todetect the content of24h urine protein and liver fatty acid-binding protein. In exploringthe protective mechanism of Bi Xie aqueous extract and main composition componen inuric acid nephropathy animal models, apply West blot method to detect the transporter,which participate in uric acid excretion and absorption, such as organic anion transporter1(OAT1), uric acid transfer factor1(URAT1) and organic cation transport factor2(OCT2).Results: Compared with control group, model group rats serum uric acid, ureanitrogen and creatinine levels were risen. Serum monocyte chemotactic protein-1andkidney tissue tumor necrosis factor-α, monocyte chemotactic protein-1and intercellularadhesion molecule-1were significantly increased. NGAL and KIM-1levels increased significantly in24h urine. Compared with model group, low dose, middle dose and highdose drug intervention group rats serum uric acid, urea nitrogen and creatinine levels werereduced. Serum monocyte chemotactic protein-1and kidney tissue tumor necrosis factor-α,monocyte chemotactic protein-1and intercellular adhesion molecule-1were significantlydecreased. The content of NGAL and KIM-1in24h urine was significantly reduced.Applied compound Bi Xie soup in Uric acid nephropathy patients show compound Bi Xiesoup can reduce the content of urine protein and Liver fatty acid-binding protein in24hurine, the effect is better than allopurinol. In exploring the protective mechanism of Bi Xieaqueous extract and main composition componen in uric acid nephropathy animal modelfound low dose, middle dose and high dose drug intervention group can promote theexpression of mOAT1mOCT2and inhibit the expression of mURAT1.Conclusion: Bi Xie aqueous extract can reduce uric acid nephropathy model ratserum monocyte chemotactic protein-1and inhibit the expression of inflammatory factor inkidney tissue can delay the progress of kidney damage and can protect the kidney.Compound Bi Xie soup can not only protect renal function of uric acid nephropathy rats,inhibit renal fibrosis, but also can obviously raise the clinical effect of Uric acidnephropathy patients. Bi Xie aqueous extract and main composition component throughraising the expression of mOAT1and reducing the expression of mURAT1to promote uricacid excretion, and raising the expression of mOCT2to lower serum creatinine levels, playa role of kidney protection. |
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