Acylation Stimulating Protein Levels And Gene Polymorphism Were Associated With Blood Pressure,Blood Glucose And Blood Lipids In Han, Uygur, Kazak Population In Xinjiang

Objective:1) To study the prevalence of hypertension, diabetes and dyslipidemia among Han, Uygur and Kazak population in Ili, Altay, Hotan Prefecture of Xinjiang.2) To investigate the mechanisms of fat hormone acylation stimulating protein (ASP) level differences among Han, Uygur, Kazak healthy adults in Xinjiang and to provide clues for further study of ethnic susceptibility to specific disorders and specific dysfunction.3) To explore the relationship between ASP gene polymorphism and blood pressure, blood glucose, blood lipid in Han, Uygur, Kazak population in Xinjiang. Method:1) Multi-stage stratified cluster random sampling method was used to select the Han, Uygur and Kazak adults over the age of35years in Hotan, Ili, Altay Prefecture in the southern and northern part of Xinjiang.7496Han, Uygur and Kazak subjects were investigated in this present study. The prevalence of overweight, obesity, hypertension, diabetes and dyslipidemia were analyzed in different ethnic, gender and age group.The risk factors of hypertension and diabetes were investigated.2) The subjects were selected from the first part of the epidemiological investigation study.331people were finally included. They were divided into three groups according to ethnics:Han group:107cases; Uygur group:134cases; Kazak group:90cases. The ASP levels changes and mechanism in normal weight healthy people were analyzed, their association with lipids, glucose, insulin and inflammatory factors were also studied.3) Subjects were selected from the first part of the epidemiological survey, the population samples were3455cases, they were divided into hypertension group and control group. The restriction fragment length polymorphism method was used for genotyping of ASP, the distribution of ASP gene polymorphism in different ethnics and their association with blood pressure, blood glucose and blood lipids were studied. Results:1) The prevalence of overweight, obesity, hypertension, diabetes in Han, Uygur, Kazak adults in Xinjiang were higher than the national average. The prevalence of hypertension and diabetes increased with age. Age, body mass index, diabetes, hypertriglyceridemia, hypercholesterolemia were the risk factors of hypertension. Age, gender, body mass index, hypertension, hypertriglyceridemia, hypercholesterolemia were risk factors of diabetes. There were ethnic, gender differences in prevalence of overweight, obesity, hypertension and diabetes. Triglycerides, total cholesterol, high density lipoprotein cholesterol levels were higher than the national level, there were ethnic, gender differences in the levels of triglycerides.2) In study subjects with age of35～60years old,23kg/m2≤BMI＜24kg/m2, normal blood glucose, normal blood lipids, normal blood pressure, normal serum uric acid, ASP levels in Uygurs were significantly lower than those in Hans. The free fatty acid levels in Uygurs were significantly higher than those in Hans. The complement C3levels in Uygur people were significantly higher than those in Han and Kazak people. The%ASP/C3in Uygur population was significantly lower than that Han and Kazak population. Plasma ASP levels were positively correlated with age,%ASP/C3and glucose in Han population. Plasma ASP levels in Uygur population were positively correlated with age, gender, systolic blood pressure, diastolic blood pressure, triglycerides, total cholesterol. It was negatively correlated with insulin levels. The important factors affecting plasma levels of ASP in Han population were insulin, body mass index, ankle-brachial index, uric acid. The important factors affecting the level of plasma ASP in Uygur population were gender, pulse wave velocity, uric acid. The important factors affecting plasma ASP levels in Kazak population were C3,%ASP/C3.3) There was no significant difference in ASP gene genotype frequency distributions between hypertension group and the control group in Hans. Among the hypertension groups in Uygurs and Kazaks, CC and CT genotype frequency were significantly increased compared the control group. The glucose, uric acid, triglyceride, cholesterol, high density lipoprotein cholesterol, low-density lipoprotein cholesterol were significant increased in Han individuals who carried C allele (CC and CT genotype). The glucose and triglyceride were significant increased in Uygur and Kazak individuals who carried C allele (CC and CT genotype). Logistic regression analysis reveals that the risk of hypertension was1.807times and0.753times higher in individuals carrying CC genotype and CT genotype respectively, than that carrying TT genotype in Uygurs. In Kazaks the risk of hypertension was1.186times and0.845times higher in individuals carrying CC genotype and CT genotype respectively, than that carrying TT genotype. Conclusions:1) The prevalences of overweight, obesity, hypertension, diabetes, dyslipidemia were higher than the national average in Han, Uygur, Kazak adults in Xinjiang. There were ethnic, gender differences.2) ASP levels were different in normal weight healthy people of different nationality, they were related to serum lipids, blood glucose, insulin and inflammatory cytokines.3) ASP gene301T>C polymorphism had the certain relevance with blood pressure, lipids, glucose, in Han, Uygur and Kazak populations of Xinjiang. C allele may be a risk factor for hypertension.