Study Oil The Molecular Mechanism Of Malignant Melanoma Metastasis By High-throughput Trancriptome Analysis

The fatality rate of Malignant Melanoma spikes once metastasis occurs. Tobetter understand the tumorigenesis and metastasis of Malignant Melanoma, weanalyzed the transcriptomes of three cell lines that correspond to three distinct stagesof Malignant Melanoma pathogenesis: the normal stage (HEMn-LP), the onset ofMalignant Melanoma (A375), and the metastasis stage (A2058).Objection:1We use the RNA-seq to get the whole map of MalignantMelanoma’s transcriptomes.2We set up a mathymatic model of MalignantMelanoma analysis based on their expression patterns by genes cluster.3In order toverify the RNA-seq data and method of analysis, the ability of migration andinvasion of Twist1, CTGF and MAGEA1overexpressed in A375were decteted.Mathod:1. Using next-generation sequencing (NGS) technology, we detectedgene expression of HEMn-LP, A37and A2058.2Gene expression data wereanalysised by IDEG6０DAVID０GO０KEGG and IPA et al.3Genes wereclustered into41categories based on their expression patterns and their biologicalfunctions were analyzed using Ingenuity Pathway Analysis.4The ability ofmigration and invasion were dected by overexpression of Twist1, CTGF andMAGEA1in A375.Results:1. The whole maps of three cell lines’(HEMn-LP、A375and A2058)transcriptomes were set up by NGS.2Gene expression level on chromosomes9,11,12, and14were non-uniform during tumorigenesis and metastasis.3Genes inscUscU category were more greatly associated with cancer metastasis than others byour41categories.4The overexpression of Twist1, CTGF and MAGEA1in A375can improve the migration and invasion of A375cells greatly.Conclusion: We analyzed the RNA-seq data of melanoma growth and metastasis. Gene expression level on chromosomes9,11,12, and14werenon-uniform during tumorigenesis and metastasis, which means their deviations maybe associated with cancer progression.2We subdivided the RNA-seq data into41categories; and found that genes in scUscU category were more greatly associatedwith cancer than others.3Twist1, CTGF and MAGEA1were selected to be ourcanidates and could improve the migration and invasion of A375cells greatly asoverexpression in the cells.