Research On The Chemical Constituents Of The Stems Of Schisandra Chinensis And Pesticidal Activities

Schisandraceae belongs to dicotyledoneae magnoliidae illiciales, includes two genera: Kadsura Kaempf. ex Juss.and Schisandra Michx.. The plants are woody stems, distribute disjunctly in Southeast Asia and the southern part of North America. There is rich Schisandraceae plants resource in China where two genera are produced, mainly in the south and southwest.In order to find new active ingredients and parts from Schisandraceae, the stems of Schsinadra chinensis(Turcz.) Baill were selected for research on the chemical constituents. 37 compounds were isolated from the ethanol extracts of the stems of S.chinensis by the system solvent extraction, Solvent step extraction, silica gel column chromatography, polyamide column chromatography, preparative thin layer chromatography, C18 column chromatography, sephadex LH-20 column chromatography, Semi-preparative chromatography, high performance liquid chromatography, recrystallization, et al. 35 of them were identified on the basis of spectral analysis(including UV, MS and NMR). There are 4 triterpenes: 24-Methylenecycloart-3-one(1), Schizandronic acid(2), Kadsuric acid(3), Oleanolic acid(4); 15 lignans: Gomisin A(5), Gomisin M1(6), Gomisin M2(7), Gomisin D(8), Schisantherin B(9), Schisanhenol(10), Gomisin J(11), Gomisin N(12), Gomisin G(13), Schisandrin B(14), Schisandrin C(15), Deoxyschisandrin(16), Gomisin C(17), Gomisin K3(18), Schizandrin(19); 9 flavonoids: Quercertin(20), Quercertin-3-O-β-D-Glucopyranoside(21), Quercetin-3-β-D-xylopyranoside(22), Rutin(23), Apigenin(24), Apigenin-7-O-β-D-glucopyranoside(25), Isorhamnetin-3-O-β-D-glucopyranoside(26), Genistein-7-O-β-D-glucopyranoside(27),(+)-Catechin-7-O-β-D-glucopyranoside(28); 3 phenolic acids: Chlorogenic acid(29), Ferulaic acid(30), Caffeic acid(31); 4 other types of compounds. 22, 26, 27 and 28 which were the first time isolated from the plant.Pesticidal activities and repellent effects of oil, fruits and stems extracts of S.chinensis against Myzus persicae and Plutella xylostella were determined. The results showed that all the extracts had Pesticidal activities and repellent effects against Plutella xylostella at 2000 mg / L, Pesticidal activities of OB, FBU, FW, SET, SBU were more than 80%; Repellent effects OB, F, SPE, SET, SW were more than 80%. Insecticide activities of all the extracts against Myzus persicae were poor, only SPE reached 60%; All the extracts against Myzus persicae have repellent effect, OL, FW, SBU reached more than 60%. pesticidal effects and repellent effects of the rest of the extracts were poor.Pesticidal effect of Schisandrol A against Myzus persicae 48 hours was 80.5%, repellent effects of Schisantherin B, Gomisin J,(+)-anwulignan, Schisantherin 和 Schisandrol A reached more than 80%. pesticidal effect of Schisandrin B against Plutella xylostella 72 hours was 100%; repellent effect of compounds were poor.LC50 value of Schisandrin B against Plutella xylostella was 586.22 mg/L, significantly lower than the Pesticidal effect of Chlorpyrifos 211.16 mg / L. LC50 value of Schisandrol A against Myzus persicae was 295.62 mg/L, significantly lower than the Pesticidal effect of Veratrine 35.29 mg/L. The results showed that pesticidal effect were good only when compounds had higher concentration, overall toxic effect was not high, compared with other botanical pesticides.8 kinds of decolorizing agents(activated clay,activated carbon,diatomite,calcium bentonite,kaolin,activated aluminium oxide,magnesium oxide,attapulgite clay)were selected,and the decolourization rates of the samples of the fruit and stem of S. chinensis and the retention rates of lignans and total triterpenes in such samples were calculated respectively.The attapulgite clay and activated carbon have the best decolourization effects on the extracts from the fruit and stem of S. chinensis respectively,and the retention rate of lignans and total triterpenes were both higher. Comparative study on the decolorizing effect of ion exchange resins: D296R、D152、D280、122、001×16、D941, in order to select the most suitable decolorant for fruit extract of S. chinensis. Decoloring solution of fruit from S. chinensis was determined by UV-VIS spectrophotometry. The content of schizandrin was determined by HPLC, the decolorization rate and retention rate of schizandrin was calculated. The results showed that D941 had the best decolorization effect for the fruit extract solution of S. chinensis.