Application Of Genome Selection In Breeding Of Zhikong Scallop(Chlamys Farreri)

Zhikong Scallop (Chlamys farreri) is an important marine shellfish aquaculture, has a high nutritional value and economic value. In this study the scallop select group of three successive generations.A large number of SNP loci are developed in three populatinos with the second-generation sequencing platform and 2b-RAD techniques.Population genetic analysis,Genome selection and Genome-wide association study are commenced in scallop to estimate breeding value, QTL fine positioning. This study will provide valuable aquaculture, especially shellfish breeding experience.1 Construction of the continuous selective populationThis chapter focuses on the construction of three generations of continuous scallop select groups. Using a truncated method with a capacity of 2000 scallop population to shell height for the top 5% on selected traits to choose, after two years of sexual maturity continued to shell height as a candidate trait selection method using a truncated until the third generation after a year long, measuring their body size traits, picked cheek wire to prepare for DNA extraction. In the selection process, the progeny of genetic traits in 24 months body size is not obvious.2 Population genetic analysis in three continuous selective populationUsing 2b-RAD sequencing technology to build a library of scallop populations in three generations of a total of 539 scallops, return data received 18,840 SNP markers. Three generations of population genetics studies found that as generations of progressive, groups marked deviation from HW equilibrium ratio of 10.34%,12.64%、 23.11%, Shannon diversity index was 0.3034 ± 0.2075,0.2963 ± 0.2138,0.2968 ± 0.1997, inbreeding coefficient for 0.0256,0.0323,0.0468. By constructing molecular phylogenetic relationship matrix found no obvious massive gathering, Fst test no significant population differentiation, linkage disequilibrium in the genome-wide detection of discovery with generations of selection choice, LD decay occurred. This shows that the continuous selection of population genetic structure Penglai red scallop species breeding population had an impact, but the genetic diversity remained at a high level, can continue breeding populations.3 Application of Genome selection an Genome-wide association analysis in scallop.Four genome selection method,GBLUP, Bayes lasso, Bayes A and Bayes B,are carried out to calculate estimate breeding value of 539 scallop individual and estimated marker effect. The results showed that four kinds of methods to estimate the distribution of marks roughly the same effect can be unified highest estimated breeding values of individuals, marking effect estimated breeding estimation results relatively uniform. With shell height for the purpose of traits Genome-wide association study, were found in two generations 20,10 a specific site. Improve the whole genome selection breeding system architecture platform for LAMP (Linux+Apache+MySQL+PHP), to achieve a rapid calculation of the estimated breeding value, more suitable for actual production.