| Objective:Psoriasis is a common chronic inflammatory skin diseases, This disorder is characterized by epidermal hyperplasia, inflammatory cell infiltration. Upregulation expression of adhesion molecules play a very important role in the infiltration of inflammatory cells to the skin in the pathogenesis of psoriasis. Serum levels of soluble adhesion molecules reflects the expression status of adhesion molecules.Recently, The effects of narrow-band ultraviolet B (NB-UVB) phototherapy in the treatment of psoriasis vulgaris have been proven and this phototherapy has become popular as routine phototherapy in the treatment of psoriasis in many countries around the world. There is little published on the impact of the NB-UVB phototherapy on the serum levels of soluable adhesion molecules in patients with psoriasis vulgaris. The aim of the present study was to investigate the effect of the narrowband UVB phototherapy on serum levels of sE-selectin, sP-selectin, sL-selectin and sICAM-1 in patients with psoriasis vulgaris. Meanwhile, the effect of the narrowband UVB phototherapy on the expression of E-selectin of HUVEC was also observed.Method:The serum levels of sE-selectin, sP-selectin, sL-selectin and sICAM-1 measured by the ELISA technique in patients with psoriasis vulgaris before and after narrow-band UVB phototherapy. It is assessed by ELISA technique that influence of narrow-band UVB irradiation on the level of soluble E-selectin released by HUVEC stimulated with TNF-a at different time; It is assessed by western blot technique that influence of narrow-band UVB on the expression of E-selectin by HUVEC six hours after stimulated with TNF-a; It is assessed by RT-PCR technique that influence of narrow-band UVB on the expression of E-selectin mRNA by HUVEC six hours after stimulated with TNF-a. Results:1 After narrow-band UVB phototherapy, the symptoms of patients with psoriasis vulgaris were significantly improved (Before treatment:PASI= 23.70±10.14; After treatment PASI= 6.02±2.07). The serum level of sE-selectin after NB-UVB treatment decreased significantly; but the serum levels of sP-selectin, sICAM-1, sL-selectin had no significant statistical difference between before and after treatment.In addition, the significant correlations were observed between the serum levels of sICAM-1 and sE-selectin, between the serum levels of sICAM-1and PASI scores.2 Under normal condition, HUVEC secreted few soluble E selectin adhesion molecules, the different doses of NB-UVB stimulation did not induce soluble adhesion molecules E selectin secretion of HUVEC.3 After 10ng/mL TNF-a stimulation, soluble E selectin levels from culture supernatant of HUVEC were significantly increased, when different doses of NB-UVB irradiation applied, the level of soluble E selectin induced by TNF-a was significantly decreased.4 NB-UVB irradiation suppressed the secretion of sE-selectin in cultured HUVEC induced by TNF-a in a dose-dependent manner.5 Six hours after HUVEC stimulated by TNF-a, E-selectin expression of HUVEC were significantly elevated, NB-UVB irradiation significantly inhibited E-selectin expression of HUVEC induced by TNF-a.6 Six hours after HUVEC stimulated by TNF-a, the level of E-selectin gene expression was significantly elevated, NB-UVB irradiation significantly inhibited TNFa-induced E-selectin gene transcription of HUVEC.Conclusion:Narrow-band UVB irradiation significantly reduced the serum levels of soluble E selectin in patients with psoriasis vulgaris, this effect may be related to inhibition of narrow-band UVB on expression of E selectin on vascular endothelial cell stimulated by inflammatory cytokines. |
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