Effect And Mechanism Of Methylhydroxy Chalcone Polymer On Diabetic Kindey Disease Rats

mesangial cell proliferation cultured in high glucose.Methods:The rat mesangial cells were incubated with different concentration of MHCP (0mg/L,10mg/L,20mg/L,50mg/L,100mg/L,200 mg/L) and glucose for 12,24 and 36 hours in vitro.Then, the proliferation activity of the cells was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) reduction assay. the proliferative index (PI) were assessed by flow cytometry after 24 hours.Results:MHCP at the concentration (20mg/L-200mg/L) was able to inhibit the proliferation of mesangial cells in a dose-dependent manner.Conclusions:Methylhydroxy Chalcone Polymer (MHCP) could inhibit the rat mesangial cell growth.Key words:Cassia; Mesangial cell; Cell proliferation; cell cyclePartâ…¢The mechanisms why Methylhydroxy Chalcone Polymer on rat mesangial cells incubated with high concentration glucoseObjective:To evaluate mechanisms why Methylhydroxy Chalcone Polymer (MHCP) on rat mesangial cells incubated with high concentration glucose.Methods:Rat mesangial cells (MCs) were cultured. When MCs grew to a single layer or partly mixed together, divided them into MHCP groups and control groups randomly. Rat mesangial cells (MCs) were stimulated with MHCP at different concentrations and times to evaluate the influence of MHCP on VEGFmRNA expression. We also investigated the change of VEGFmRNA and Protein, Collagenâ…£, the content of Nitric Oxide Synthase (NOS) and Nitric Oxide (NO) in the process of co-incubation of mesangial cells with MHCP and glucose for 24 hours.VEGFmRNA was measured by semi-quantification Reverse Transcription Polymerase Chain Reaction (RT-PCR), VEGF cellular protein content in cell culture media was detected by Enzyme-linked immunosorbent assay (ELISA) quantitative analysis, Collagenâ…£in cell culture media were measured by Western blotting.Results:The expressions of VEGFmRNA and protein treated by MHCP(200mg/L) are 1.11-fold and 1.09-fold greater respectively than control groups. The expression of Collagen IV and the content of NO and NOS treated by MHCP (200mg/L) were obviously decreased which are 1.14-fold,1.4-fold and 1.05-fold greater respectively than control groups in the media.The changes were related with the concentrations of MHCP which was a concentration-dependent manner.Except of except Oh,the expression of VEGFmRNA,VEGF and Collagenâ…£was gradually decreased by time length(4h,8h,16h,24h) when mesangial cells exposed to MHCP(100mg/L), there were time responses in respose to increasing time incubated with MHCP and high glucose.Conclusion:MHCP abolished high glucose-stimulated VEGFmRNA, VEGF expression by the dose response of the NO-stimulated promoter activity in response to increasing concentrations of MHCP in mesangial cells, then less the lesion of kindey by significantly decreasing expression of Collagenâ…£.