Study On The Qingrejiedu Therapy Of Chronic Inflammation And Related Changes

Research purposesThe purpose of the theory part in this study is to investigate the relevance of chronic inflammation and the toxicity and heat.the chronic inflammatory disease-related drugs from the heat of the idea of governance;summary of the use of heat-clearing and detoxifying treatment of chronic inflammatory diseases related to the treatment of the law,for clinical differentiation therapy in this provide a theoretical basis for diseases.Experimental part of one of chronic inflammatory disease-related angiogenesis as a target to study the heat-clearing and detoxifying the representative major components of Chinese medicine baicalin baicalin,honeysuckle main component of chlorogenic acid on the impact of the incident,so as to provide clinical experimental basis for the clearing away heat and toxic method to provide a scientific explanation of real support.Research MethodsTheoretical part of research-oriented literature,a comprehensive summary of the results of previous studies,the depth of excavation in the Western theory of internal relations in different fields,into a pathology and treatment of common law.Experimental program is divided into two parts,in vivo and in vitro,in vivo portion of chicken chorioallantoic membrane(CAM) as a model to observe the drug of baicalin,chlorogenic acid on the impact of the role of angiogenesis;vitro mainly through the observation of baicalin,chlorogenic acid on the different aspects of angiogenesis(vascular endothelial basement membrane degradation,endothelial cell proliferation,migration and lumen formation) effect,to evaluate whether a drug of anti-angiogenesis activity.Specific methods for:Ea.hy926 of vascular endothelial cells to conventional training,the use of MTT assay of drugs on cell proliferation;scratch experiments, Transwell experiments to observe the drug on endothelial cell migration;artificial plastic matrix observed drugs on endothelial cells lumen formation;gelatin zymography detection of drugs on endothelial cell secretion of MMP9,MMP2 activity.Experimental data using statistical software SPSS for processing.The resultsIn this study,in theory,chronic inflammation of the connotation of a comprehensive summary of the latest follow-up study at home and abroad,focusing on the chronic inflammatory disease,especially related to angiogenesis in the background;from the inside heat-related toxicity and chronic inflammatory disease of the respective characteristics carried out on the relevance of the two,summed up the internal links and the law;and thus further discusses the use of heat-clearing and detoxifying treatment of chronic inflammatory disease of drug-related law.Part of the experiment are summarized as follows:1,in vivo experiments found that the CAM method,regardless of baicalin or chlorogenic acid,various concentrations of a certain degree of angiogenesis inhibition,and baicalin(0.01,0.1,1 mM concentration,respectively,the inhibitory rate for 53.33%,66.67%,73.33%) showed dose-dependent manner.2,MTT experiment showed that baicalin Ea.hy926 can inhibit the proliferation,OD values compared with the blank control group were significantly different(p＜0.01),various concentrations(1, 0.1,0.01 mM) under the cell survival rate was 49.976%,70.78%,71.01%;high concentration of chlorogenic acid(1 mM) could inhibit cell proliferation,OD values compared with the blank control group were significantly different(p＜0.05),but no dose-dependent manner.3, experiments on the scratch cell migration rate in each group statistical data,compared to curcumin,various concentrations of baicalin(1,0.1,0.01 mM) migration rates were 20.67%, 58.67%,97.33%,showed more obvious inhibition;various concentrations of chlorogenic acid (1,0.1,0.01 mM) migration rates were 60.67%,1.0067%,1.2467%,a certain degree of concentration-dependent.4,Transwell experiments showed that baicalin group migrated cells were 29±4,41.8±17.12,36.7342.8±6.98,76.6±7.74,compared with the control group with significant difference(p＜0.01),chlorogenic acid in cell migration group were 11.8±3.03,26.8±5.68,85.6±13.24,99±7.88,compared with the control group with significant difference (p＜0.01).5,in the endothelial tubule formation assay,the high concentration of baicalin, chlorogenic acid(1mM) on endothelial Ea.hy926 tubule formation and fracture with the role of inhibition.6,gelatin zymography experiments,Baicalin Ea.hy926 effectively inhibited the secretion of collagenase MMP-9,MMP-2,thereby inhibiting migration of endothelial cells, with inhibition of MMP-2 most significant,and a clear dose-dependent manner.Chlorogenic acid in high concentrations(1mM) significantly reduced MMP9,2 bands of negative staining, no obvious difference in low concentration.