The Effect Of Shou' Erzhi Capsule On The Ion Channel Of The Alzheimer Disease And The Hippocampus Neurons Cells

Objective: the patch clamp technique in the whole cell recording was used to study the effect of Shou' erzhi capsule on the hippocampus neurons cells of healthy rats and ion channel expressed by AD(Alzheimer disease) model rats' cerebral neurotransmitters. Based on these experiments, the preventive and therapeutic mechanism of Alzheimer's disease with traditional Chinese herbs has been studied.Methods: (1) prepared rabbits medicated serum by serum pharmacological method, and been divided into three groups, including Shou' erzhi medicated serum, Haboine medicated serum and physiological saline medicated serum (blank group for short). In Shou' erzhi medicated serum group, ligustrazine (components of Shou' erzhi capsule, and the effective composition of ligustra) was chosen to carry out qualitative detection as representative.(2) Dispersed and cultivated hippocampus neurons cells by utilizing neurons cells dispersion and culture techniques, which would be used to record ion channel current in the patch clamp technique and the whole cell recording.(3) Applying ion channel principle and the patch clamp technique in the whole cell recording to record the Na ion, K ion and L-Ca ion channel currents of hippocampus neurons cells (with medicated serum) respectively, and observing the changing of its I-V curves.(4) Replicated AD model rats by using D- galactose taking inject ion method after eye's ball, recorded the neurotransmitters channel current and transmembrane voltage of AD model rats' cerebral mRNA expressed in xenopus oocyte, and observed the influences of Shou' erzhi medicated serum on transmembrane voltage and channel current.Results: (1) The qualitative detection of ligustrazine showed rabbits serum contained the drug ingredients of Shou' erzhi herbs.(2) After separation, cultured hippocampus neurons cells grew well. Hippocampus neurons cells, cultured 12-15 days, attach wall completely, had greater cell volume, and was used to high resistance seals, and could observe synapse spontaneous activities remarkably in cell record.(3) Shou' erzhi medicated serum could enhance the peak value of Na ion channel current effectively, accelerate Na ion channel inactivation; and also increase the peak value of K ion current obviously, reinforce K ion channel conductance; and inhibit the peak value of L- Ca current effectively, which has significant differences compared to blank group. ( p<0.05)(4) Shou' erzhi medicated serum could strengthen transmembrane voltage and channel current of AD model rats' cerebral mRNA expressed in xenopus oocyte, which has obviously discrepancies compared to blank group. ( p<0. 05)Conclusions: This research suggested that Shou' erzhi capsule has apparent influences on the Na ion, K ion and L-Ca ion channel current and relative I-V curves of rats' hippocampus neurons cells. Chinese herbal compound Shou' erzhi could increase peak value of Na ion and K ion current (I_Na, I_k) apparently, and accelerate Na ion channel inactivation simultaneously. Then it also could reinforce K ion channel conductance, shorten depolarization time of hippocampus neurons, and generate strong amplitude action potential rapidly. Besides, it restrained hippocampus neurons cells depolarization continually and shortened its repolarization time. Shou' erzhi inhibited the peak amplitude of L- Ca current effectively. Hence, it blocked Ca ion internal flow, and decreased Ca content in the cell to prevent apoptosis caused by Ca overload.By researching the effect of Shou' erzhi on ion channel current expressed by AD model rats' cerebral neurotransmitters, the results showed Shou' erzhi can promote excitatory neurotransmitter release. Therefore, Shou' erzhi could both stimulate the neurons cells and inhibit the excitatory neurotransmitter release excessively, through action mechanism mentioned above. Thereamong, accelerating Na ion channel inactivation and blocking Ca ion internal flow were the main functions to avoid cells damage by excitation toxic effect for neurotransmitter release excessively, which would prolong cells activities. Hence, Shou' erzhi had definite functions to protect brain tissue and improve memory, aiming to prevent and treat Alzheimer' s dementia.