Experimental Study On Treatment Of Gastric Cancer With Human CIK Cells Transfected With Human IL-15 Gene

In this study,we investigated the therapeutic efficacy of the human CIK cells transfected by hIL-15-IRES-TK retroviral vector in human gastric cancer cell models which are human low - differentiation gastric cancer cell line BGC-823 to cell immune-deficient mice(Balb / c-nu / nu mice).we constructedy hIL-15-IRES-TK retroviral vectorT cells transfeced by hIL-15-IRES-TK retroviral vector can secrete IL-15.In view of autoimmune diseases are potentially dangerous by adoptive transferation of T cells,thymidine kinase gene(HSV-TK) were expressed in T-lymphocytes which can be removed by ganciclovir(GCV).CIK ceils were transfected by hIL 15-IRES-TK plasmid,hIL-15-IRES-TK retroviral transfectied CIKcells and non-transfected CIK cells were local injected to tumor of mices respectively,once a week,total two weeks.Mices were observed two weeks after treatment.physiological saline were local injectied as a control group.Tumor volumes in mices were measured weekly.Tumor tissue of mices were weighed out after 4 weeks.In treatment period,the effect of hIL-15-IRES-TK plasmid transfected and non-transfected CIK cells showed a significant difference at second week.It showed that the L-15-IRES-TK plasmid transfected CIK cells have a stronger antitumor effect than non-transfected CIK cells.In 3rd and 4th week,tumor volumes of three groups of mices were increased,but they are smaller in groups of IL-15-IRES-TK plasmid transfected and non-transfected CIK cells than in the saline group.At same time,statistical differences of tumor volumes was detected between the groups of hIL-15-IRES-TK plasmid transfected and non-transfected CIK cells at observation period.It showed that persistence time of antitumor reactivity of CIK cells can be lengthened significant after transfected by hIL-15-IRES-TK plasmid.In the experimental process,2 mices in IL-15 transfected CIK Group showed tumors fester after the beginning of the treatment,then ulcer healed and tumor disappeared at fourth week.Other two group mices does not have this phenomenon.This is also indirectly reflected the L-15-IRES-TK plasmid transfected CIK cells have a stronger antitumor effect than non-transfected CIK cells.The results showed that CIK cells can effectively inhibit growth of gastric cancer cell line BGC-823,antitumor reactivity of CIK cells can be enhanced significant after transfected by hIL-15-IRES-TK plasmid.