Extraction Of Polysaccharide From Huidouba And Its Hypoglycemia Activity

Huidouba, which is an effective Chinese traditional medicine to treat the diabete in local, is the cobweb that secreted by parasitic spider on the tree at an elevation of 1500m on Emei mountain in Sichuan province. The extraction process of Huidouba polysaccharide and its anti-diabetic activity were studied in this work. There is no report on this field until now.The method to induce the diabetic animals was investigated at first. After the animals were feed for 3 days and drunk sugar water, the freshly prepared solutions of Alloxan (140mg/kg body weight) wereintraperitoneally injected to the 16 hours fasted mice, while 120mg/kg first day and 100mg/kg next day for rats. Three hours after that, the animals were fed with 25% glucose solution(1ml/100g), then, the blood glucose levels were tested with blood sample from orbital venous plexus. The animal was selected as diabetic model whose glucose level was higher than 11.1mM and sustained at that level. In the period of feeding, fresh water and clean living conditions must be guaranteed. paddings were changed every two days and water was supplied every day. Animals were kept in a special animal house at an ambient temperature of 22±1℃and 50-65% relative humidity. The induction rate was connected with the amount of Alloxan, injection way and feeding method.The chemical identification results indecate that Huidouba consists polysaccharide and no protein, Huidouba may contain steroid, phenols, tannins, saponins and alkaloids. So, we extracted crude polysaccharide in traditional method. In our research, the Huidouba polysaccharide (HDBW-1) could decrease the blood glucose level of alloxan-induced diabetic mice by 44.3% and decrease the blood glucose level of alloxan-induced diabetic rats by 42.4%. Significantly, the hypoglycemic effect of Huidouba polysaccharide(HDBW-1) is superior to that of Huidouba ethanol extract(HDBE) and Huidouba water extract(HDBW).Moreover, the Huidouba polysaccharide exhibits no obvious hypoglycemic effects on normal mice or rats(P>0.05). So we could draw the conclusion that the effective component of Huidouba is polysaccharide(HDBW-1).Huidouba polysaccharide was extracted in this work. The extraction process was optimizated by response surface method. I studied the effect of Huidouba polysaccharide on diabetic animals. The results show that Huidouba polysacharide has obvious hypocemic effects on diabetic animals. Furthermore, It is shown that the anti-oxidation and lipid lowing activities increased from the changes of SOD,GSH-Px,MDA,TG,TC,LDL-C,HDL-C,BUN after treatment with Huidouba polysaccharide. The healthy animals were fed by Huidouba polysaccharide for 30 days, then, the animals was induced to diabete by Alloxan. There is no significant difference between the blood glucose levels before and after induction. It is shown that the Huidouba polysaccharide could prevent the diabete. In another experiment, the results showed that the Huidouba polysaccharide could treat the diabete because the glucose level of diabetic animals decreased after they were fed with Huidouba polysaccharide. It is shown that the anti-oxidation and lipid lowing activities increased from the changes of SOD,GSH-Px,MDA,TG,TCHO,LDL-C,HDL-C,BUN after treatment with Huidouba polysaccharide. It could be concluded that the Huidouba polysaccharide could protect isletβcell from the injury of Alloxan.Through isolation and purification, the Huidouba polysaccharide was devided into three components-HDBW-1Cα,HDBW-1Cβand HDBW-1Cγ.The monosaccharide composition of all the three components are the same-mannose, rhamnse, glucose, galactose, xylose, arabinose, fucose, glucuronic acid and galacturonic acid.At last, it is also shown there is little toxicity for Huidouba polysaccharide after the acute, sub-acute, long term toxcicity experiments and special toxcicity experiments. It would be a potential drug to treat the diabetes.