Effection Of Avastin And Triamcinolone Acetonide For Uveitis In The Rabbit's Eyes

Objective:Since the researches on the human uveitisare are subject to numerous limitations, the animal modes are used in the extensive research. There are numerous animal model of uveitis are used in study at present. In this article bovine serum albumin are used to adult melanin rabbit for immunity and experimental autoimmune uveitis models are built as the instrument of empirical study on the nosogenesis of uveitis and immunotherapy. In recent years, long-term glucocorticosteroid (triamcinolone acetonide) dissolves and exhaust lymphocytes in absorbent gland, lien and thoracic gland, especially the T lymphocytes, through lowering the body's normal immune response to restrain macrophages of its function of swallowing and handling the antigen, to reduce the function of reticuloendothelial system in clearing particulate antigen or degenerating cell. The long-term glucocorticosteroid (triamcinolone acetonide) has been extensively used in some autoimmune diseases and achieved extremely effective treatment outcome. Different styles of administration are used to cure uveitis of rabbits appraise its therapeutical effect on uveitic clinical manifestation and microscopic anatomical changes. The intraocular in some communities. Prevalence and type of the blinding disorder vary greatly depending on the areas. Nations or countries in the world. Comparision of uveitis statisties from various countries is diffcult because of diverse diagnostic criteria. Investigations and changing etiological theories. Some types of uveitis have a definite geographical distribution.Method:1. The building of rabbit uveitis model induced by BSA: 12 adult New Zealand black rabbits were chosen and divided into untreated control group and experimental group. BSA was dissolved in sterilized water for injection and brought to the concentration of 2% and 4% 0.25ml syringe was pierced into anterior chamber at an oblique angle to extract aqua oculi 0.25ml; another syringe of the same type was pierced into corporis vitre of eyeball to put 0.25ml of 4% BSA. One week later, each rabbit was injected 0.25ml of 2% BSA on the marginal ear vein. The rabbits of control group, on the other hand, were injected equal amount of sterilized normal saline. The above operations were all performed in sterile environments. With the situation under close review, the clinical manifestation of the ill rabbits were evaluated and scored clinically, and graded by histology.2. The therapeutical effect of glucocorticoid to experimental uveitis of rabbits:(1)Another 60 adult melanin rabbits were chosen and divided into two treatment groups, one is glucocorticoid treatment group and another is Avastin treatment. Each of the two treatment groups was redivided according to three treatment methods, which are subconjunctival administration, retrobulbar injection and eyeball-inside injection. The rabbits are evaluated clinically and observed from histopathology after the administration for 3, 7, 14 and 30 days.(2)The melanin rabbits of positive control group were injected BSA immune-induced uveitis through Ear marginal vein. The rabbits in the treatment group were administrated 7 days later after the immune-induced uveitis injection, when their uveitis is at the most obvious moment, while the rabbits in positive control group were given equal amount of sterilized normal salin.(3)Clinical and histological examination: after being immuned, the rabbits were received once slit lamp microscope examination and Funduscopy each day. The time of onset and apex time were detailedly recorded, including clinical clinical performance and score, and Inflammation regression time. Two groups of rabbits were executed with aeroembolism on different moment, and their eyeballs were taken for normal pathological sections, which were observed with optical microscope and evaluated by histological grade for the treatment effect of two drugs to Uveitis.3. Statistical Methods:(1)SPSS13.0software was used for statistical analysis. Simple model group and treatment group were performed different sample t-test.(2)SPSS13.0 software was used for statistical analysis. Mean and standard deviation of retinal inflammatory cells and lymphocytes of simple model group, positive control group, hormone therapy group and Avastin treatment group were calculated separately, which are in terms of x|-±s. Groups were compared through variance analysis, LSD-t test.Results:The Uveitis disease model was successfully copied from the melanin rabbits of the experimental groups, among which the attack rate is100% and all models showed typical Uveitis; on the fifth day, 15 rabbits have showed symptoms of angiectasia in tunica coerulea and conjunctival congestion, among which some showed anterior chamber flare and cells, and became heavier gradually then until inflammation reach the peak in the 7th day: blurred texture of tunica coerulea, mixed injection, pupil opisthosynechia, contracted pupil, exudation of hemaleucin within anterior chamber, pupillary closure, opacitas corporis vitrei, and eye ground that cannot be looked into. These physical signs disappeared gradually later until the 30th day, when the inflammation was subsided. From histology, stromatic infected cells in anterior and posterior chamber and corpus ciliare choroideae were infiltrated and exuded with a great quantity of infected cells aggregation within corporis vitre. The clinical situation of triamcinolone acetonide treatment group (group C) and Avastin group (group D) was lessened, whose average clinical score and histological grade bellowed the simple model group, and the comparisons are statistically significant; but the treatment effect of triamcinolone acetonide treatment group was more apparent than that of Avastin group, which are of statistical significance. There was no notable difference between positive control group (group B) and simple model group (group A), which is not statistically significant; the comparisons among subconjunctival administration (C1,D1), retrobulbar injection (C2,D2) and eyeball-inside injection (C3,D3) within treatment group were obvious, which are statistically significant; eyeball-inside injection treatment was apparently better than the other two administration methods. The comparison between subconjunctival injection and retrobulbar injection was of statistical significance as well, and retrobulbar injection was obviously better than subconjunctival injection.Conclusion:The rabbit uveitis mode induced by BSA was successfully constructed in the body of adult pigmented rabbit. The clinical manifestation of this model is similar to human uweitis, so the results can be used in the related researches on the nosogenesis and immunotherapy of human uweitis. This method is convenient, stable and has good reproducibility and high inception rate, which has provided the experimental foundation for further researches on the immunologic mechanism, prevention and treatments of uveitis.many questions remain unanswered in uveitis as far.the aetiology is complicated and the pathological mechanism is not clear up to date. Treatment of uveitis is still challenging for some cases although there are a variety of therapies available currently. Complications often occur due to unclear diagnosis and/or lack of appropriate treatments in time. Repeated recurrence leads to irreversible lesions,deterioration. it seems imperative to further investigate ITS clinical characteristics further and determine its causes further so as to give appropriate treatment and have better prognosis. to obtain systemic knowledge of the clinical characteristics of uveitis in china.Glucocorticoid can be used to heal uveitis effectively, which helps improve symptoms and delay time of onset. This experiment confirms further the important effect of injecting triamcinolone acetonide within eyeball during uveitis, which indicates that local and long-term continued injection with high concentration is an effective way to cure uveitis. Avastin also has certain effect on uveitis: probably because it indirectly promotes inflammatory cells to produce anti-angiogenic stimulating factors, prevents the migration of macrophage and labrocytes, and restrains the activity of growth factor and its related factors, which promote vasifaction, to prevent the growth of new vessels. The activity of factors related to growth factor, which promote vasifaction, is restrained to prevent the growth of new vessels. Many reports indicate that ocular toxoplasmosis is a major cause of uveitis in Europe. In the present study only one case of particular-induced uveitis was found over a thousand patients investigated. It remains to be explored further why there is such low prevalence of the parasite infection of the eye in this over-populated.