Studies On Chemical Constituents And Quality Control Methods Of RADIX PHLOMII

RADⅨPHLOMⅡis the dried root of perennial herbage Phlomis younghusbandii Mukerjee which belongs to the genus Phlomis of family Labiate. RADⅨPHLOMⅡis commonly used in Tibetan medicine named as "Lumue" in Tibet. According to traditional medical the theories of Tibetan,RADⅨPHLOMⅡtastes bitter,cool in nature and belongs to the lung,it has the functions of dispelling wind and clearing heat,relieving cough and reducing phlegm,promoting tissue regeneration and wound healing,it is used to treat common cold due to wind heat, cough with profuse sputum,pyogenic skin infection without healing for along time, etc.For further reasonable development and application,we have systematically studied on chemical constituents,pharmacological effects and quality control methods of RADⅨPHLOMⅡ.Five pharmacodynamic experiments firstly done on different extracted parts approved the acetic acid ethyl ester part and the n-butyl alcohol part of RADⅨPHLOMⅡhad prominent biological activities,such as relieving cough,reducing phlegm,appeasing asthma and anti-inflammatory.These results showed the two parts are the effective parts of RADⅨPHLOMⅡ.The chemical element study done by us indicated that RADⅨPHLOMⅡmay contains amino acid,protein,amylase,tannins,phenols,organic acid,flavonoids, coumarins,anthraquinones,triterpenes,steroids and saponins,etc.In due to find the material basis of RADⅨPHLOMⅡthat relate to pharmacodynamic action,we investigated chemical constituents of RADⅨPHLOMⅡby chemical and spectroscopic analysis.By phytochemical researches,12 compounds were isolated from the acetic acid ethyl ester of RADⅨPHLOMⅡand ten of them were identified as follows:phlomisosideⅥ(1),shanzhiside methyl ester(2),luteolin(3), kaempferol(4),berberine(5),ursolic acid(6),emodin(7),rhein(8),β-daucosterol(9),β-sitosterol(10).They were first obtained from this plant for the first time besides compound 2,7,8,among them compound 5 was isolated from the genus of Phlomis for the first time,and compound 1 was a new compound.Reference Substance solution used for the quality control of RADⅨPHLOMⅡwas firstly provided by us.After these reseaches,such as resorce,extractive technics, chemical structure confirmation,purity examination,content determination,etc.we drafted the quality standard for phlomisosideⅥ.In aspect of the quality standard for RADⅨPHLOMⅡ,the identification of characteristics,micro-construction and TLC was carried out;shanzhiside methyl ester and phlomisosideⅥwere determined by RP-HPLC for the first time;The quality standard protocol for RADⅨPHLOMⅡwere recensed and perfected on the basis of the reaseaches mentioned above.The research about fingerprint chromatogram of RADⅨPHLOMⅡshowed that under the chosen experimental conditions,chemical components of the methanol extract had similar retention times and chromatograms.This method is meaningful as a measure of quality control when the chemical components are not entirely known.In short,through the studies mentioned above on RADⅨPHLOMⅡ,it partially revealed the pharmacodynamic material basis and supplied a gap of identification of micro-construction and TLC,quantitation determination,fingerprint chromatogram. Our study provided the theoretical basis and scientific reference for the further study, development and application of RADⅨPHLOMⅡ,and had great referential significance of the modifying and consummating of the quality standard for RADⅨPHLOMⅡ.Meanwhile,these researches highly promotes modernization of Tibetan Medicine.