| Objective:Expound the biological mechanism of Radix Polygalae reducing the gastric mucosa damage by honey-fried,reveal the scientific connotation of traditional Chinese medicine reducing the stomach and intestines toxic by concoct in molecular level,It has great practical value and the academic significance of using Radix Polygalae reasonably and safely.Methods:Application method of Pharmacology,stomach and intestines electrophysiology,molecular biology and so on.Explored the crude Radix P.and its saponins and the honey-stir-baked Radix P.to the gastric mucosa damage and the protection mechanism,We did the following researches:①use helianthin to research gastric emptying;②use BL-420 biologic analyzer to observe and record the electrophysiology and gastric microcirculation;③use RIA to determine SP,SS;④use fluorescence pectrophotometriy to determine PGE2,test enzyme activity of Ca++-ATPase by the colorimetric determination of phosphorus;⑤use RT-PCR to study the expression of COX-2,CaM,α-TGF and ITF.Result:①Crude Radix P.and its saponins can obviously inhibit gastric motility(P＜0.01),remarkably both inhibit stomach's fast wave and slow wave(P＜0.01,P＜0.05),but Honey-stir-baked Radix P.can not;②Crude Radix P.can obviously decrease SP and SS(P＜0.05),Honey-stir-baked Radix P.can remarkably increase SP and decrease SS;③Crude Radix P.can remarkably cause gastric mucosal microcycle barrier(P＜0.05 );④Both Crude Radix P.and Honey-stir-baked Radix P. have not influence to PGE2;Honey-stir-baked Radix P.can enhance cox-2 expression (P＜0.05);⑤Crude Radix P.can obviously decrease the activity of Ca++-ATPase in gastric tissue and Honey-stir-baked Radix P.enhance CaM expression(P＜0.05);⑥Honey-stir-baked Radix P.can enhanceα-TGF and ITF expression(P＜0.05).Conclusion:Crude Radix P.(10g/kg) and its saponins(5g/kg) can damage the gastric mucosa,but Honey-stir-baked Radix P.can reduce this damage,The biological mechanism of Honey-stir-baked Radix P.reducing the gastric mucosal damage may related to the following factors:①Honey-stir-baked Radix P.can obviously increase SP of gastric tissue and improve the suppression of Crude Radix P. to stomach's fast wave,Thus improves the gastric motility;②Increase gastric mucosal blood flow,enhances the anti-damage and repair ability;③Improve the suppression of Crude Radix P.to the activity of Ca++-ATPase in gastric tissue, eliminates the cell "calcium overload" to the mucous membrane harm;④The promotion epithelial cell's fission and strengthening restitution of damaging region;⑤Enhancea-TGF and ITF expression,increase the gastric mucosa protection and damage repair.In brief,Honey-stir-baked Radix P.realize multi-level,the multi-ways to the gastric mucosa protection and the damage repair mainly through the promoting gastric mucosa microcycle,enhancing stomach organization local hormone release,urged the related enzyme activity,urged the gastric mucosal protection factor expression and so on. |
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