Tangzhiping On The High Fat Diet Insulin Resistance In Rats And Some Of Its Mechanism Of Action

At present,the number of diabetic patients in China are growing at an amazing rate,it is estimated that there will be 46 million diabetics over the age of 20 untill 2025,and it has seriously affected the life quality and expectancy of the patients.In China,patients with type 2 diabetes mellitus(T2DM)are the prevalence,insulin resistance(IR)is the main cause to type 2 diabetes and exists before diabetes onset,so it is significant to improve the status of IR for the prevention and treatment of IGT and T2DM,while Traditional Chinese Medicine(TCM)plays a distinct role in it.PurposeThis research is based on effective clinical treatment of IR and IGT,quoting the idea of "Zhiweibing" to IR theory and experiment research of TCM,inducing IR rats through high-fat-diet,to explore the function mechanisms,provide the basis for theories and methods of TCM treatment.Methods1 Literature Studies:Recent literature concerning type 2 diabetes,insulin resistance,adiponectin,GLUT and PPARγare documented.So this study has taken a full account of the pathogenesis mechanisms,medication,evaluation and research methods of IR into consideration both in TCM and allopathic medicine and made a systematic review of adiponectin,GLUT and PPARγadvancements.2 Theoretical Studies:To improve the status of IR with TCM under the guidance of "Zhiweibing" idea; research the Chinese medicine pathogenesis of early IR phase on the basis of existing achievements,the system type is differented into Tanyu retention,take advantage of Huatan Huoxue thrapy,and Tangzhiping is used to improve the status of IR for the prevention and treatment of IGT and T2DM.3 Laboratory Studies:The IR rats were induced by high-fat-diet and then treated them with Tangzhiping, comparing to the PPARγligand Rosiglitazone,performed the euglycemic hyperinsulin clamp technique to evaluate the insulin sensitivity,observed Tangzhiping's influence on glucose metabolism,lipid metabolism,insulin sensitivity,serum adiponectin level,GLUT4 and PPARγ2 mRNA expression of IR rats. Results1 Evaluation of Animal Model:We induced laboratory IR rats by high-fat-diet,manifesting as increasing in weight, systolic blood pressure,FINS,disorder in lipid and glucose metabolism,significantly lower glucose metabolism rate(M)than that in control group,showing remarkable IR,adding to the moderate feeding cycle,just as the incidence of human IR features,which are relatively satisfied animal model.2 Effects Evaluation and Mechanisms Research:To investigate Tangzhiping's influence on glucose,lipid metabolism as well as insulin sensitivity of IR rats,and to explore the function mechanisms from the perspective of cellular and molecular biology based on the success of experimental rat model of IR.The results show that Tangzhiping can increase glucose metabolism rate(M)(P＜0.05); decrease the weight of IR rats significantly(P＜0.01),better than that of Rosiglitazone (P＜0.01);both of Tangzhiping and Rosiglitazone can reduce systolic blood pressure,but not significantly(P＞0.05);lower fasting blood glucose in rats(P＜0.05),just as Rosiglitazone (P＞0.05);significantly reduce plasma TG and LDL-c levels(P＜0.01,P＜0.05),while TC and HDL-c little changed(P＞0.05);lower FINS(P＜0.01),just as Rosiglitazone(P＞0.05); enhance the level of serum adiponectin(P＜0.01),just as Rosiglitazone(P＞0.05);promote the expression of skeletal muscle GLUT4 mRNA and adipocyte PPARγ2 mRNA significantly (P＜0.05,P＜0.01).ConclusionBased on the above findings,we believe that Tangzhiping can improve glucose and lipid metabolism,increase insulin sensitivity,enhance serum adiponectin level,promote adipocyte differentiation factor-PPARγ2 and glucose transporter protein-GLUT4 mRNA expression significantly,which paly an important role in improving insulin sensitivity, enhancing the periphery tissue glucose uptake and utilization under insulin stimulation.