The Quality Of The Flavors Of Chinese Herbal Medicines Such As Menispermi Research, And Evaluation

Rhizoma Beidougen is rhizome of Menispermum dauricum DC. belonging to Menispermaceae.It is bitter in taste and cold-natured, it attribute to lung meridian ,stomach meridian, and large intestine meridian, it has effects of heat-clearing and detoxicating, wind-dispelling pain-relieving. It used to treatment throat pain, enteritis, rheumatism and arthralgia pain.Sarcandra glabra is entire herb of Sarcandra glabra (Thunb.)Nakai belonging to chloranthaceae. It is bitter, acrid in taste and gentle in nature, it attribute to heart meridian and liver meridian, it has effects of heat-clearing and blood-cooling, blood-activating and removing ecchymosis. It used to treatment blood -heat and purple plague, purpura, rheumatism and arthralgia pain, traumatic injury.Swertia mileensis is entire herb of Swertia mileensis T.N.Ho et W.L.Shi belonging to gentianaceae. It is bitter, sweet in flavor and cold in nature, it attribute to hepatobiliary meridian, which has effects of liver-clearing and cholagogic, heat-clearing and dampness-removing. It used to treatment jaundice and red urine, hot stranguria and pain acerbity.Folium Syringae is leaf of Syrin ga obl ate Lindl. belonging to Oleaceae .It is bitter in flavor and cold in nature. It has effects of heat-clearing and toxicity-removing, antiphlogistic and dysentery -stopping.Rabdosia rubescens is drying leaf and aerial part of Rabdosia rubescens (Hemsl.) Hara belon- ging to Labiatae,which harvested in summer and autumn, dried in the sun. It is bitter and sweet in flavor, gentle in nature. It effects of heat-clearing and toxicity-removing, blood-activating to arrest pain. It used to treatment throat pain, tonsillitis, insect and snake biting.This topic is divided four parts.The first part: Determination of five traditional Chinese medicines such as Rhizoma Beidougen index components. Determination of five traditional Chinese medicines such as Rhizoma Beidougen from different collection places by HPLC. Determination of sinomenine (first report), daurisoline (first report), dauricine and tetrandrine (first report) in Rhizoma Beidougen; Determination of fumaric acid, isofraxidin, astilbin (first report) of Sarcandra glabra; Determination of sweaiamarin, gentiopicroside (first report), sweroside (first report), oleanolicacid (first report) of Swertia mileensis; Determination of protocatechuic acid, tyrosol (first report), caffeic acid (first report) of Folium Syringae; Determination of oridonin, rosmarinic acid, caffeic acid (first report) of Rabdosia rubescens.The second part: Application of switching wavelength technology on determination of five traditional Chinese medicines such as Rhizoma Beidougen by HPLC. Because many kinds of components existed in Chinese medicine, each component cannot be detected simultaneously by the sole wavelength detection, enable the major part experiment only to carry on the determination in an analysis to some ingredient. While achieving simultaneous determination of the mult-index components in the highly sensitive by switching wavelength technology of HPLC, which can complete in a joint operation that normally detected under the conditions of several experiments. this part first use this technology to complete simultaneity determination of sinomenine, daurisoline, dauricine and tetrandrinein Rhizoma Beidougen; Determination of fumaric acid, isofraxidin, astilbinof Sarcandra glabra; Determination of sweaiamarin,gentiopicrosidet,sweroside in Swertia mileensis; Determination of protocatechuic acid, tyrosol in Folium Syringae; Determination of oridonin, rosmarinic acid in Rabdosia rubescens.The third part: Quality evaluation research of five traditional Chinese medicines such as Rhizoma Beidougen. We combined determination of each index components of five traditional Chinese medicines such as Rhizoma Beidougen by HPLC, used identify methods of grey recognition pattern recognition method, established recognition model of quality grey recognition pattern above five traditional Chinese medicines (first report), and proposes their determination standards separately. It provided new and effective method for traditional Chinese medicine quality control and synthetic evaluation.The fourth part: Conclusion. This topic has conducted the quality research to five traditional Chinese medicines, innovatively used wave length-switching technology for determination of the traditional Chinese medicine multi-components by HPLC. Use the data-mining technology, according to method of gray relevance analysis pattern, carried on the traditional Chinese medicine quality rating pattern the research, the purpose is to summarize the rule, for comprehensive promote traditional Chinese medicine quality specification, provide the experience for the traditional Chinese medicine quality control's application of the modern new technology .