Depression In Rats. Light Capsule Intervention Mechanism Of Sex Hormones On Rat Brain Stem The Tph <sub> 2 </ Sub> Gene Expression

Objective:The objective of this dissertation is to analyze the relationship between sex hormones and depression,to investigate intervention mechanisms of Jingqing capsules through the rat models of premenstrual depression state caused by exogenous high-dose-estrogen,to Explore inflection of sex hormone for rats' behaviors and 5-HT and gene expression of TPH₂ in brain stem,and analyze the relationship between menstrual depression and high-level-estrogen by injecting different sex hormones into different sex experimental rats.Methods:We used animal model of three to four months old impregnated SD female and male rats, performed vaginal smear test for each female rats and set up the files of estrous cycle individually.We Choose rats according with demands,randomly divided the rats into eight different experimental groups,female rats contrast group,male rats contrast group,female rats Estradiol group,male rats Estradiol group,the reserpine model group,female rats Estradiol with Jingqing Capsule group,male rats Estradiol with Jingqing Capsule group,the reserpine model with Jingqing Capsule group.We injected Estradiol Benzoate into experimental rats on three consecutive mornings to introduce high-level-estrogen state,made the model during the estrous period.At the same time of modeling, given Jingqing Capsule for different group and observed changes of rats' behavioral,Estradiol, progestin,testosterone as well as changes of 5-HT and gene expression of TPH₂ in brain stem.Choose rats according with demands in the Second experiment,Randomly divided the rats into six different experimental groups:female rats contrast group,female rats Estradiol group,female rats progestagen group,female rats androgenic hormones group,.male rats contrast group,.male male Estradiol group. Given exogenous estrogen,progestagen and androgenic hormones on three consecutive mornings (during the estrous period of female).Observed changes of rats' behavioral changes as well as changes of 5-HT and gene expression of TPH₂ in brain stem.Results:high-dose-estrogen can decrease response degree to external stimulant,shorten rats' covered distance of y + labyrinth test,decrease the 5-HT content in brain stem,increase gene expression of TPH₂.Experiments rats are slowmoving, response is indifferent,body is soft and no resistance.These results show that exogenous high-dose-estrogen can cause depression in experimental rats when given during the late estrous period.These were also found in reserpine model group.Qingjing capsules can reduce depression in the animal,increase rats' covered distance of y + labyrinth test,decrease level of E₂.increase 5-HT content in the brain stem,decrease gene expression of TPH₂.High-dose-androgenic can increase rats' behavior total grade,decrease 5-HTgrey-number in the brain stem,decrease gene expression of TPH₂.These deferens between estradiol and androgenic hormones are obvious.Conclusion: Exogenous high-dose-estradiol during the estrous period can cause the premenstrual depression state in experimental rats,Jingqing capsules can reduce the pathological change,and hence the animal's depression can be relieved.Exogenous high-dose-estradiol can decrease experimental rats' quantities of neurotransmitter in the brain stem,hence experimental rats show depression.High-dose-androgenic hormones can increase rats' quantities of neurotransmitter in the brain stem,so experimental rats show excitation.