Umbilical Cord Mesenchymal Stem Cells In The Hgf Gene Transfection On Liver Injury Regeneration Research

Backgrounds and objective:The regeneration and recovery of liver after cell damage has been a fairly important issue in this field.One of the most promising therapeutic approaches is to use stem cell transplantation,but the resources of the stem cells limit its further application.Mesenchymal stem cell is a type of somatic stem cell and is widely distributed.It demonstrated high ability of immunosuppression and could inhibit the function of NK cell,T lymph cell,B lymph cell and dendrite cell.Therefore,it is the most popular stem cells which can be genetic modified before transplantation. Mesenchymal stem cell can derive from the matrix cell in the umbilical cord(UC-MSCs), which make it even convenient to get.However,there is no report regarding the induction of UC-MSCs into liver cell.The purpose of this study is to observe the therapeutic function of HGF transgenic UC-MSCs in liver damage rat model.Methods:Mesenchymal stem cell was induced from the matrix cell in the umbilical cord and followed by transfection of HGF expressing lentivirus vector.The rat model was established by partial liver resection plus a single intraperitoneal CCL4 injection.The genetic modified stem cells were transplanted through the hepatic vein.The regeneration and recovery of the liver cells was evaluated by monitoring the following parameters:the liver function by the serum enzyme level,the histopathological change of the hepatic cell by HE staining,the growth of UC-MSCs by immunostaining,and the localization of the UC-MSCs by detecting of HGF expression.Results:The HGF transgenic mesenchymal stem cell and liver damage rat model were successfully established.After transplantation,the stem cells could be positively detected in the damaged liver cells and these stem cells could locally express HGE Compared with the control group,the treated group showed an improved morphological and functional phenotype based on the serum enzyme level and pathological examination.Conclusion:The matrix cell in the umbilical cord provides an easy way to obtain mesenchymal stem cell and lentivirus can successfully deliver HGF expression vector into UC-MSCs.Moreover,partial liver resection plus a single intraperitoneal CCL4 injection is a better approach to establish liver damage rat model.And the most important is that UC-MSCs could get to the damaged liver,express HGF and help the regeneration and recovery of the liver cells.The detailed mechanism still remains to be clarified.