The Effect Of Calcium Sulphate On Bone Reconstruction And Preparation Of Calcium Sulfate/Platelet-rich Plasma Bioactive Scaffold

Because of trauma, especially high-energy trauma, arthroplasty revision surgery, removal of bone tumor causes massive bone defect, and the patients need for bone substitute has been increasing greatly. Commercial calcium sulfate is favored for its biocompatibility and completely degradation in body. The speed of degradation rate is accord with that of new bone formation. At present, China's no SFDA approved surgical calcium sulfate. We prepared surgical-grade a-calcium sulfate with citric acid which has good biocompatibility and can be absorbed along with its degradation. Compression technique was used to increase the compressive strength of the calcium sulfate and coating technique was used to control the degradation speed of calcium sulfate. We prepare delayed release materials by conbined calcium sulphate with BMP and antibiotics. Although calcium sulfate is a sort of biocompatible, biodegradable material which has good compressive strength, as a scaffold, we know little about its mechanim of reconstruction of bone defects. Calcium sulphate conbined with BMP has both bone conduction and bone induction ability, but expensive. The study was designed to find the effect of calcium sulphate on bone reconstruction and preparation of calcium sulfate/platelet-rich plasma bioactive scaffold for bone regeneration.PurposeTo find the effect of calcium sulphate on bone reconstruction and prepare calcium sulfate /platelet-rich plasma bioactive scaffold for bone regeneration.Materials and methodsCulture bone marrow stromal stem cells and research the role of calcium sulphate in chematropism of BMSCs; investigate the effect of calcium sulphate on the gene expression of BMSCs by the second generation of gene chip technology during bone marrow stromal stem cells induced into osteoblast-lieke cells. Prepare platelet-rich plasma and calcium sulfate compound scaffold, and research its physico-chemical properties and biocompatibility. Restoring the segmental bone defect of rabbit with the scaffold and observing the osteoinduction role of the material.ResultsCalcium sulfate of bone marrow stem cells have the role of Chemokines in human bone marrow stromal stem cells. Calcium sulphate significantly change the gene exression during the process of the cells induced into osteoblasts. Quantitative functional gene chip can detect all currently known osteoblast-related genes. 89 genes were detected in this experiment. 23 kinds of them change significantly (> 2-fold). Compared with the contrast group, 14 genes of the experimental group increased more than two times: AMELY: 2.11; BMP2: 3.35; COL4A3: 3.64; COMP: 6.70; EGF: 2.70; FLT1: 3.10; IGF1: 5.79; ITGA2: 2.18; MMP10: 13.12; MMP2:2.11; TGFB2: 2.52; TGFBR1: 2.82; VDR: 2.63; VEGFA: 2.61. 9 genes reduced more than two-fold: COL2A1: -2.23; COL15A1: -2.47; COL1A1: -8.37; COL1A2: -2.17; COL5A1: -2.09; CSF2: -3.6; FGF1: -2.86; ITGA3: -5.41; MMP8: -5.05. Calcium Sulfate / platelet-rich plasma has stable craystle and high compressive strength, up to 38.5 MPa, which similar to cancellous bone. Completely degradation time in vitro is about 41 days. Calcium sulfate combined with platelet-rich plasma can induce bone marrow stromal stem cells to osteoblast-like cells and induce ectopic ossification in nude mice. Biological activity of calcium sulfate was greatly improved by combing with platelet-rich plasma. It can be used to restore critical bone defec of rabbit radial. It is an ideal bone substitute with bone induction and conduction.ConclusionCalcium sulfate of bone marrow stem cells have the role of Chemokines in human bone marrow stromal stem cells. Calcium sulphate significantly change the gene exression during the process of the cells induced into osteoblasts. Calcium sulphate has potential bone induction ability. Calcium Sulfate/platelet-rich plasma is an ideal bone substitute with bone induction and conduction.