Protein Expression Profiling Of Mouse Ovarian Build And Polycystic Ovary Syndrome Proteomics Research

Ovary is the major female gonad with responsibility for producingestrogen, progesterone and oocyte. A complete understanding of proteinsexpressed in the mouse ovary will provide biological information onmechanism underlying oogenesis and female reproductive regulation. Thepurposes of this study were to apply a proteomic approach to investigatingprotein composition and to establish a 2-D PAGE reference map for mouseovarian proteins. When total protein was assayed by 2-D PAGE andstained with sliver, about 2000 protein spots with a pI of pH 3-10 and Mrof 12-120 kDa could be detected. All these protein spots were excised foridentification, among which 213 spots corresponding to 192 differentproteins were identified undoubtful by searching the mass spectra againstthe SwissProt/TrEMBL database. A 2-D PAGE-based mouse ovarianprotein database has been constructed on the basis of these results. All theidentified proteins were bioinformatically annotated in detail based ontheir molecular function, biological process etc. About 80% of theseproteins play roles in generalized metabolism process. Ovariancharacteristic metabolic pathways have been analysed according to Keggpathway databases. There are more than 20 other metabolic pathways thatexist in mouse ovary such as glycolysis pathway and oxidativephosphorylation metabolism pathway. Further, Except for participatingin metabolic pathways, the proteins expressed in mouse ovary also havesome other important functions, for example, binding protein and nucleotide, having hydrolase and oxireductase activities, binding ion andso on. Using PathwayAssist software, we also established mouse ovarianproteomic complex analysis, the protein regulated by a great amount ofproteins was the interleukin family, which hinted that this family hasimportant role in the ovary. Through this research, we got comprehensiveknowledge of proteins existed in mouse ovary, this information should bevaluable for improving our understanding of oogenesis, folliculardevelopment, ovarian physiology, and physiopathology. Besides thisanalysis, we have done comparative proteomics analysis of normal andpathologic ovaries, ovaries of Polycystic ovary syndrome patients wereselected for analysis, Several proteins exhibited significantly differentexpressions have been found, these proteins may play roles in the processof oogenesis and follicular development. Immunohistochemical analysesof some of them, including HSP27, HSP10 and HSP47, were performed.Further studies of these proteins would be helpful to illustrate molecularmechanism of oogenesis and follicular development, merit clinicaldiagnosis and provide clues for important insight for a betterunderstanding of the pathogenetic mechanisms underlying this clinicaldisorder.