In-depth Study Of The Active Ingredients Of Tcm Small Root Garlic

Allium macrostemon Bunge is widely distributed in the northeast of China. Its dried bulbs were popularly known as traditional Chinese medicine "Xiebai" which can be used for treatment of some heart diseases such as throracic pain, stenocardia, heart asthma, etc. In our previous research, 13 new steroidal saponins were found from this plant by bioactivity-guided isolation and some of them presented strong inhibitory activity on ADP-induced platelet aggregation, such as Macrostmonoside A, E and F.Through our research work, 36 compounds were isolated from the 60% ethanol extract of the dried bulbs of Allium Macrostemon B. The structures of them have been elucidated as stigmasterol (1), Adenosine (2) , Macrostemonoside E (3),(25S)-26-O-β-D-glucopyranosyl-5α-furost-20 (22)-ene-3β 26-diol-3-O-β-D-glucopyranosyl (1→2) [β-D-glucopyranosyl (1→3)]-β-D-glucopyranosyl (1→4)-β-D-galactopyranoside (4), (25S)-26-O-β-D-glucopyranosyl- -5β-furostane-3β, 22, 26-triol-3-O-β-D-glucopyranosyl (1→2)-β-D-galactopyranoside (5) , (25R)-26-O-β-D-glucopyranosyl-5β-furostane-3β, 22, 26-triol-3-O-β-D-glucopyranosyl (1→2)-β-D-galactopyranoside (6) ,(25R)-26-O-β-D-glucopyranosyl-22-methoxy-5β-furostane-3β, 26-diol-3-O-β-D-glucopyranosyl (1→2)-β-D-galactopyranoside (7), Macrostemonoside B (8), Macrostemonoside C (9), (25R)26-O-β-D-glucopyranosyl-furost-5 (6)-ene-3β,22,26-triol-3-O-β-D-glucopyranosyl (1→2) [β-D-glucopyranosyl (1→3)J-β-D-glucopyranosyl (1→4)-β-D-galactopyranoside (10) , Macrostemonoside F (11) , Macrostemonoside G (12) , Macrostemonoside H (13) , Macrostemonoside I (14) , 26-O-β-D-glucopyranosyl-5β-furost-12-one-3β, 22, 26-triol-3-O-β-D-glucopyranosyl (1→2)-β-D-galactopyranoside (15), (22S)-cholest-5-ene-1-β, 3β, 16β, 22-tetraol-1-O-α-L-rhamnopyranoside-16-O-β-D-glucopyranoside (16) , (25R)-26-O-β-D-glucopyranosyl-5α-furostane-2α, 3β, 22, 26-tetraol-3-O-β-D-glucopyranosyl (1→2) [β-D-glucopyranosyl (1→3)]-β-D-glucopyranosyl (1→4)-β-D-galactopyranoside (17)*, (25S)-26-O-β-D-glucopyranosyl-5β-furostane-1β, 3β, 22, 26-tetraol-3-O-β-D-glucopyranosyl (1→2)-β-D-galactopyranoside (18)*, 26-O-β-D-glucopyranosyl-5β-furost-25 (27)-ene-3β, 22, 26-triol-3-O-β-D-glucopyranosyl (1→2)-β-D-galactopyranoside (19)*, (25R)-5β-furostane-1β, 2β, 3β, 6α, 22-pentaol-26-O-β-D-glucopyranoside (20)*, 5β-furost-25 (27)-ene-1β, 2β, 3β, 6a, 22-pentaol-26-O-β-D-glucopyranoside (21)*, Macrostemonoside J (22), Macrostemonoside K...