Obese Adipose Tissue Gene Expression Profiles Characteristic And Human Obesity-related Novel Gene C10orf116 Function

With the aim of identifying novel molecular pathways in human obesity, suppression subtractive hybridization (SSH) was performed in our study.Non-obese subjects and obese patients without any other diseases, such as endocrine diseases, metabolism diseases, cardiovascular diseases, malignant diseases and chronic inflammation, were recruited and the differentially expressed genes in omental adipose tissue were screened. Insulin resistance, as the common complication of obesity, was analyzed and excluded more strictly. Since the two groups of subjects have comparable state except weight and BMI, any differences in gene expression between groups are most likely due to obesity. In our study 426 genes were screened as the potential obesity-related genes, 216 obese up-regulated gene and 210 normal up-regulated gene. Several obesity related genes, such as ACRP30, Glut4, IGF1, LPL and aP2, were also screened in this study and in so doing served as compelling controls for the SSH procedure. To further verify and validate the SSH results, we performed RT-PCR analysis of 10 randomly chosen differentially expressed genes. Overall, the expression patterns obtained by RT-PCR reflected the results obtained by SSH, demonstrating the low false positive rate associated with SSH. 232 genes were identical to sequences that had previously been identified and characterized, among which, 174 genes had functional research, while 58 had no functional research. Besides, 158 genes were identical to previously identified...