Patients With Acute Coronary Syndrome Angiogenesis And Circulating Endothelial Cells

Objective In the first part: (1) To study the concentration variety of vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1) and soluble Tie-2 (sTie-2) in patients with acute myocardial infarction (AMI), a condition whose pathophysiology also involves angiogenesis. (2) To investigate the influence of recombinant VEGF, Ang-1 and Tie/Fc chimera on apoptosis and tube formation of human umbilical endothelial cells (HUVECs) and to investigate the possible signaling pathway; (3) To establish and investigate the feasibility of a new system, the optical proteinchip based on imaging ellipsometry (OPC-IE), assaying the serum level of sTie-2. In the second part: (1) Isolate the circulating endothelial cells (CECs) from AMI and UA patients by using the dynabeads coated with anti-CD 146 and (2) to study the relationship among CECs, C-reactive protein (CRP), interleukin-6 (IL-6) and tumor necrotic factor (TNFα); (3) According to the results of these two parts, the relationship between serum level of sTie-2 and endothelium injury were investigated.Methods (1) Levels of VEGF, Ang-1 and sTie-2 in 27 patients with AMI at different time after administration and 28 healthy controls were measured by ELISA; (2) MTT, Hoechst33342 and Annexin V/PI kit were used to assay the effects of recombinant VEGF, Ang-1 and Tie-2/Fc on apoptosis of HUVECs; (3) HUVECs were seeded in three-dimensional fibrin gel and treated with recombinant Ang-1 or Tie-2/Fc chimera. Tube formation area was measured after 48 hours, and (4) the expression of phospho-Tie2 and phospho-Akt were tested by western blotting; the concentration of NO in supernatant was measured by enzymatic method; (5) Established the sTie-2 detecting biosensor, and the serum levels of sTie-2 were measured on OPC-IE system; (6) 37 patients with AMI, 12 patients with UA, and 42 health controls were studied; CECs were isolated from peripheral blood by using of immunomagnetic beads coated with antibodies against CD146. Their endothelial origin was confirmed by the positive labelling of von Willebrand Factor (vWF),...