| 1. Establishment the model of isolated lung transplantation in rabbitObjective: To establish a simple, reliable lung transplantation model and introduce the application of the model in the study of lung preservation. Methods: The donor lungs of rabbits were flushed with the low-potassium-dextran ( LPD ) solution through the main pulmonary artery , and stored at 4°C for 16 hours . The cyclic perfusion were performed in a humidified, temperature-controlled ( 37°C ) chamber. The donor lungs were ventilated with 100%O2 at a rate of 40 breaths.min-1 with a tidal volume of 25 ml and reperfused with venous blood from right atrium of recipient rabbits. Blood gas analysis of inflow and outflow blood was performed at 10 min intervals and continuous mean pulmonary artery pressure ( PaP ) and peak airway pressure ( PawP ) were monitored. Results: The recipient rabbit could provide reliable venous blood ( PvO2 = 36.55-38.32 mmHg PHv = 7.35-7.38 ). The cyclic perfusion course lasted 1 hour and the donor lungs could oxygenate well ( PaO2=130.53-198.90mmHg ) and maintain good compliance (PaP = 41.95-80.07 mmHg, PawP = 12.95-18.64 mmHg ) in the first 30min . But in the last 30min,the donor lung's ability of gas exchange and compliance was worse ( PaO2= 63.75-92.18 mmHg, PaP = 41.95 - 80.07 mmHg, PawP = 12.95-18.64 mmHg ) ( p<0.001 ) . Conclusion: This isolated lung transplantation model could imitate the procedure of lung transplantation reliably and be applied in the study of lung preservation and transplantation.2. Improved lung function by means of retrograde flush in theisolated lung transplantation in rabbitsObjective : Retrograde flush of preservation solutions has the theoretic advantage of achieving homogeneous distribution in the lung because of perfusing both the pulmonary and the bronchial circulation. This study was to evaluate the effects of retrograde flush via the left atrium in the isolated rabbits lung transplantation and... |
PDF Full Text Request